Novel splice variants of amphiphysin I are expressed in retina

Yoshiko Terada, Ken Tsutsui, Kuniaki Sano, Osamu Hosoya, Hiroshi Ohtsuki, Akira Tokunaga, Kimiko Tsutsui

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Using RT-PCR-based cDNA cloning, we identified novel splice variants of amphiphysin I, termed amph Ir, that are expressed specifically in retina. In comparison with the prototype amphiphysin I, amph Ir contained two novel insertions (inserts A and B) and one deletion. Insert A is only 9 bp in length but appears to be a determinant for the retina-specific expression. In contrast, insert B is a large domain of 1740 bp and two shorter transcripts with 3′-truncated insert B were also expressed. All the insert sequences were present as unidentified exons in the amphiphysin I gene on human chromosome 7. Western blot analysis of various rat tissues with anti-insert B antibody confirmed the presence and tissue specificity of the variant proteins.

Original languageEnglish
Pages (from-to)185-190
Number of pages6
JournalFEBS Letters
Volume519
Issue number1-3
DOIs
Publication statusPublished - May 22 2002

Fingerprint

Retina
Tissue
Organ Specificity
Chromosomes, Human, Pair 7
Cloning
Human Chromosomes
Chromosomes
Rats
Organism Cloning
Exons
Complementary DNA
Genes
Western Blotting
Polymerase Chain Reaction
Antibodies
amphiphysin
Proteins

Keywords

  • Amphiphysin
  • Retina
  • Splice variant

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Terada, Y., Tsutsui, K., Sano, K., Hosoya, O., Ohtsuki, H., Tokunaga, A., & Tsutsui, K. (2002). Novel splice variants of amphiphysin I are expressed in retina. FEBS Letters, 519(1-3), 185-190. https://doi.org/10.1016/S0014-5793(02)02763-1

Novel splice variants of amphiphysin I are expressed in retina. / Terada, Yoshiko; Tsutsui, Ken; Sano, Kuniaki; Hosoya, Osamu; Ohtsuki, Hiroshi; Tokunaga, Akira; Tsutsui, Kimiko.

In: FEBS Letters, Vol. 519, No. 1-3, 22.05.2002, p. 185-190.

Research output: Contribution to journalArticle

Terada, Y, Tsutsui, K, Sano, K, Hosoya, O, Ohtsuki, H, Tokunaga, A & Tsutsui, K 2002, 'Novel splice variants of amphiphysin I are expressed in retina', FEBS Letters, vol. 519, no. 1-3, pp. 185-190. https://doi.org/10.1016/S0014-5793(02)02763-1
Terada, Yoshiko ; Tsutsui, Ken ; Sano, Kuniaki ; Hosoya, Osamu ; Ohtsuki, Hiroshi ; Tokunaga, Akira ; Tsutsui, Kimiko. / Novel splice variants of amphiphysin I are expressed in retina. In: FEBS Letters. 2002 ; Vol. 519, No. 1-3. pp. 185-190.
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AU - Tsutsui, Kimiko

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