Novel single-chain variant of antibody against mesothelin established by phage library

Hiromasa Yakushiji, Kazuko Kobayashi, Fumiaki Takenaka, Yoshiro Kishi, Midori Shinohara, Masaru Akehi, Takanori Sasaki, Eiji Ohno, Eiji Matsuura

Research output: Contribution to journalArticle

Abstract

Mesothelin (MSLN) shows increased expression in various cancer cells. For clinical application of antibodies as a positron emission tomography (PET) imaging reagent, a human shortened antibody is essential both for avoiding redundant immune responses and for providing rapid imaging. Therefore, we cloned a single-chain fragment of variable regions (scFv) from a human-derived gene sequence. This was achieved through the construction of a naïve phage library derived from human tonsil lymphocytes. Using a column with human recombinant MSLN, we carried out bio-panning of phage-variants by colony formation. We first obtained 120 clones that were subjected to selection in an ELISA using human recombinant MSLN as a solid phase antigen, and 15 phage clones of scFv with a different sequence were selected and investigated by flow cytometry (FCM). Then, six variants were selected and the individual scFv gene was synthesized in the VL and VH domains and expressed in Chinese hamster ovary cells. Mammalian cell-derived human-origin scFv clones were analyzed by FCM again, and one MSLN highly specific scFv clone was established. PET imaging by 89Zr-labeled scFv was done in mice bearing xenografts with MSLN-expressing cancer cells, and tumor legions were successfully visualized. The scFv variant established in the present study may be potentially useful for cancer diagnosis by PET imaging.

Original languageEnglish
Pages (from-to)2722-2733
Number of pages12
JournalCancer Science
Volume110
Issue number9
DOIs
Publication statusPublished - Sep 1 2019

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Single-Chain Antibodies
Bacteriophages
Clone Cells
Positron-Emission Tomography
Neoplasms
Flow Cytometry
Antibodies
Palatine Tonsil
Cricetulus
mesothelin
Heterografts
Genes
Ovary
Enzyme-Linked Immunosorbent Assay
Lymphocytes
Antigens

Keywords

  • Zr (zirconium-89)
  • mesothelin
  • phage library
  • positron emission tomography (PET) imaging
  • single-chain fragment of variable region

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Novel single-chain variant of antibody against mesothelin established by phage library. / Yakushiji, Hiromasa; Kobayashi, Kazuko; Takenaka, Fumiaki; Kishi, Yoshiro; Shinohara, Midori; Akehi, Masaru; Sasaki, Takanori; Ohno, Eiji; Matsuura, Eiji.

In: Cancer Science, Vol. 110, No. 9, 01.09.2019, p. 2722-2733.

Research output: Contribution to journalArticle

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