Novel restriction fragment length polymorphism of the growth hormone gene in inbred rats

Tetsuo Kunieda, Hiroshi Ikadai, Minami Matsui, Nobuo Nomura, Tomonori Imamichi, Ryotaro Ishizaki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A novel restriction fragment length polymorphism in inbred rats was detected by Southern blot analysis with rat growth hormone cDNA as a probe. Four alleles, characterized by PstI fragments of 1.2, 1.1, 0.9, and 0.7 kb, respectively, were detected in 27 strains examined. The same distribution of polymorphisms was observed on digestion of DNAs of these strains with three other enzymes, PvuII, HindIII, and BamHI. Moreover, the same differences in length of allelic restriction fragments were obtained with these restriction enzymes as with PstI. These findings suggested that the polymorphism was caused by insertion or deletion of variable DNA segments in the second intron of the growth hormone gene. Linkage analyses using backcross progeny provided no evidence for close linkage between the restriction fragment length polymorphism locus and 10 other loci examined.

Original languageEnglish
Pages (from-to)591-602
Number of pages12
JournalBiochemical Genetics
Volume27
Issue number9-10
DOIs
Publication statusPublished - Jan 1 1989
Externally publishedYes

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Keywords

  • growth hormone gene
  • mammalian genetics
  • Rattus norvegicus
  • restriction fragment length polymorphism

ASJC Scopus subject areas

  • Biochemistry
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

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