Novel recombinant BCG and DNA-vaccination against tuberculosis in a cynomolgus monkey model

Yoko Kita, Takao Tanaka, Shigeto Yoshida, Naoya Oohara, Yasufumi Kaneda, Sachiko Kuwayama, Yumiko Muraki, Noriko Kanamaru, Satomi Hashimoto, Hiroko Takai, Chika Okada, Yukari Fukunaga, Yayoi Sakaguchi, Izumi Furukawa, Kyoko Yamada, Yoshikazu Inoue, Yuji Takemoto, Mariko Naito, Takeshi Yamada, Makoto MatsumotoDavid N. McMurray, E. C. Dela Cruz, E. V. Tan, R. M. Abalos, J. A. Burgos, Robert Gelber, Yasir Skeiky, Steven Reed, Mitsunori Sakatani, Masaji Okada

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62 Citations (Scopus)

Abstract

We have developed two novel tuberculosis (TB) vaccines: a DNA vaccine combination expressing mycobacterial heat shock protein 65 (Hsp65) and interleukin-12 (IL-12) by using the hemagglutinating virus of Japan (HVJ)-liposome (HSP65 + IL-12/HVJ) and a recombinant BCG harboring the 72f fusion gene (72f rBCG). These vaccines provide remarkable protective efficacy in mouse and guinea pig models, as compared to the current by available BCG vaccine. In the present study, we extended our studies to a cynomolgus monkey model, which is currently the best animal model of human tuberculosis, to evaluate the HSP65 + IL-12/HVJ and 72f rBCG vaccines. Vaccination with HSP65 + IL-12/HVJ as well as 72f rBCG vaccines provided better protective efficacy as assessed by the Erythrocyte Sedimentation Rate, chest X-ray findings and immune responses than BCG. Most importantly, HSP65 + IL-12/HVJ resulted in an increased survival for over a year. This is the first report of successful DNA vaccination and recombinant BCG vaccination against M. tuberculosis in the monkey model.

Original languageEnglish
Pages (from-to)2132-2135
Number of pages4
JournalVaccine
Volume23
Issue number17-18
DOIs
Publication statusPublished - Mar 18 2005
Externally publishedYes

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Keywords

  • HSP65 DNA + IL-12 DNA vaccine
  • Monkey
  • Tuberculosis

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Kita, Y., Tanaka, T., Yoshida, S., Oohara, N., Kaneda, Y., Kuwayama, S., Muraki, Y., Kanamaru, N., Hashimoto, S., Takai, H., Okada, C., Fukunaga, Y., Sakaguchi, Y., Furukawa, I., Yamada, K., Inoue, Y., Takemoto, Y., Naito, M., Yamada, T., ... Okada, M. (2005). Novel recombinant BCG and DNA-vaccination against tuberculosis in a cynomolgus monkey model. Vaccine, 23(17-18), 2132-2135. https://doi.org/10.1016/j.vaccine.2005.01.057