Novel NTP binding property of rice dwarf phytoreovirus minor core protein P5

Nobuhiro Suzuki, Tomonobu Kusano, Yoshiharu Matsuura, Toshihiro Omura

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Rice dwarf phytoreovirus (RDV) mRNA synthesized from purified virion has a cap structure, m7GpppAm-, which suggests the presence of guanylyltransferase activity in the virion. We attempted to identify the enzyme involved in the cap formation by using a nucleoside triphosphate binding assay. Incubation of virion with [α-32P]GTP resulted in labeling of an 89-kDa protein that had not previously been identified in purified virus preparations. Interestingly this protein also covalently bound UTP and ATP, which is not a property of the known guanylyltransferases. RDV particles catalyzed GTP-PP(i), dGTP-PP(i), ATP-PP(i), and UTP-PP(i) exchange reactions. In SDS-polyacrylamide gel electrophoresis, the 89-kDa protein comigrated with the SE-coded protein, P5, which had been expressed by a baculovirus vector. Moreover, the labeled 89-kDa protein was precipitated by an antiserum against this recombinant RDV P5. Careful reinvestigation of purified virus particles by SDS-polyacrylamide gel electrophoresis and Western blotting analyses showed that they contained a small amount of P5 (

Original languageEnglish
Pages (from-to)471-474
Number of pages4
JournalVirology
Volume219
Issue number2
DOIs
Publication statusPublished - May 15 1996
Externally publishedYes

Fingerprint

Reoviridae
Virion
Uridine Triphosphate
Guanosine Triphosphate
Proteins
Polyacrylamide Gel Electrophoresis
Adenosine Triphosphate
Baculoviridae
Nucleosides
Immune Sera
Western Blotting
Oryza
Viruses
Messenger RNA
Enzymes

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Novel NTP binding property of rice dwarf phytoreovirus minor core protein P5. / Suzuki, Nobuhiro; Kusano, Tomonobu; Matsuura, Yoshiharu; Omura, Toshihiro.

In: Virology, Vol. 219, No. 2, 15.05.1996, p. 471-474.

Research output: Contribution to journalArticle

Suzuki, Nobuhiro ; Kusano, Tomonobu ; Matsuura, Yoshiharu ; Omura, Toshihiro. / Novel NTP binding property of rice dwarf phytoreovirus minor core protein P5. In: Virology. 1996 ; Vol. 219, No. 2. pp. 471-474.
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