Novel intracellular effects of human connective tissue growth factor expressed in Cos-7 cells

Satoshi Kubota, Takako Hattori, Tsuyoshi Shimo, Tohru Nakanishi, Masaharu Takigawa

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

To clarify the multiple functionality of connective tissue growth factor (CTGF), we examined the effects of nascent CTGF within the cell by transient expression. In Cos-7 cells, expression of human CTGF induced an altered cell morphology. It was associated with an increased cellular DNA content and loose attachment, indicating the cells were in G2/M phase. Overexpression of CTGF did not induce cell growth, whereas recombinant CTGF efficiently stimulated the proliferation extracellularly. These results indicate that intracellular CTGF may act as an antimitotic agent, thus it should also be noted that nascent CTGF was found to accumulate around the central mitotic machinery. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)58-62
Number of pages5
JournalFEBS Letters
Volume474
Issue number1
DOIs
Publication statusPublished - May 26 2000

Keywords

  • Cell cycle arrest
  • Chondrocyte
  • Connective tissue growth factor

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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