Novel intracellular effects of human connective tissue growth factor expressed in Cos-7 cells

Satoshi Kubota; Takako Hattori; Tsuyoshi Shimo; Tohru Nakanishi; Masaharu Takigawa

doi:10.1016/S0014-5793(00)01573-8

Novel intracellular effects of human connective tissue growth factor expressed in Cos-7 cells

Satoshi Kubota, Takako Hattori, Tsuyoshi Shimo, Tohru Nakanishi, Masaharu Takigawa

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

To clarify the multiple functionality of connective tissue growth factor (CTGF), we examined the effects of nascent CTGF within the cell by transient expression. In Cos-7 cells, expression of human CTGF induced an altered cell morphology. It was associated with an increased cellular DNA content and loose attachment, indicating the cells were in G2/M phase. Overexpression of CTGF did not induce cell growth, whereas recombinant CTGF efficiently stimulated the proliferation extracellularly. These results indicate that intracellular CTGF may act as an antimitotic agent, thus it should also be noted that nascent CTGF was found to accumulate around the central mitotic machinery. Copyright (C) 2000 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	58-62
Number of pages	5
Journal	FEBS Letters
Volume	474
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(00)01573-8
Publication status	Published - May 26 2000

Keywords

Cell cycle arrest
Chondrocyte
Connective tissue growth factor

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(00)01573-8

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title = "Novel intracellular effects of human connective tissue growth factor expressed in Cos-7 cells",

abstract = "To clarify the multiple functionality of connective tissue growth factor (CTGF), we examined the effects of nascent CTGF within the cell by transient expression. In Cos-7 cells, expression of human CTGF induced an altered cell morphology. It was associated with an increased cellular DNA content and loose attachment, indicating the cells were in G2/M phase. Overexpression of CTGF did not induce cell growth, whereas recombinant CTGF efficiently stimulated the proliferation extracellularly. These results indicate that intracellular CTGF may act as an antimitotic agent, thus it should also be noted that nascent CTGF was found to accumulate around the central mitotic machinery. Copyright (C) 2000 Federation of European Biochemical Societies.",

keywords = "Cell cycle arrest, Chondrocyte, Connective tissue growth factor",

author = "Satoshi Kubota and Takako Hattori and Tsuyoshi Shimo and Tohru Nakanishi and Masaharu Takigawa",

note = "Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan (T.N., M.T.) and the Ministry of Health and Welfare′s Research Grant for Specific Diseases, Japan (M.T.), and grants from the foundation for Growth Science in Japan (M.T.), the Sumitomo Foundation (M.T.) and Research for the Future Programme of The Japan Society for the Promotion of Science (JSPS) (Project: Biological Tissue Engineering, JSPS-RFTF98I00201). The authors wish to thank Drs. Takashi Nishida, Masahiro Asano and Gen Yosimichi for helpful suggestions, Ms. Masako Hata and Ms. Kazumi Oyama for technical assistance, and Ms. Etsuko Fujisawa for secretarial assistance.",

year = "2000",

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doi = "10.1016/S0014-5793(00)01573-8",

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AU - Kubota, Satoshi

AU - Hattori, Takako

AU - Shimo, Tsuyoshi

AU - Nakanishi, Tohru

AU - Takigawa, Masaharu

N1 - Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan (T.N., M.T.) and the Ministry of Health and Welfare′s Research Grant for Specific Diseases, Japan (M.T.), and grants from the foundation for Growth Science in Japan (M.T.), the Sumitomo Foundation (M.T.) and Research for the Future Programme of The Japan Society for the Promotion of Science (JSPS) (Project: Biological Tissue Engineering, JSPS-RFTF98I00201). The authors wish to thank Drs. Takashi Nishida, Masahiro Asano and Gen Yosimichi for helpful suggestions, Ms. Masako Hata and Ms. Kazumi Oyama for technical assistance, and Ms. Etsuko Fujisawa for secretarial assistance.

PY - 2000/5/26

Y1 - 2000/5/26

N2 - To clarify the multiple functionality of connective tissue growth factor (CTGF), we examined the effects of nascent CTGF within the cell by transient expression. In Cos-7 cells, expression of human CTGF induced an altered cell morphology. It was associated with an increased cellular DNA content and loose attachment, indicating the cells were in G2/M phase. Overexpression of CTGF did not induce cell growth, whereas recombinant CTGF efficiently stimulated the proliferation extracellularly. These results indicate that intracellular CTGF may act as an antimitotic agent, thus it should also be noted that nascent CTGF was found to accumulate around the central mitotic machinery. Copyright (C) 2000 Federation of European Biochemical Societies.

AB - To clarify the multiple functionality of connective tissue growth factor (CTGF), we examined the effects of nascent CTGF within the cell by transient expression. In Cos-7 cells, expression of human CTGF induced an altered cell morphology. It was associated with an increased cellular DNA content and loose attachment, indicating the cells were in G2/M phase. Overexpression of CTGF did not induce cell growth, whereas recombinant CTGF efficiently stimulated the proliferation extracellularly. These results indicate that intracellular CTGF may act as an antimitotic agent, thus it should also be noted that nascent CTGF was found to accumulate around the central mitotic machinery. Copyright (C) 2000 Federation of European Biochemical Societies.

KW - Cell cycle arrest

KW - Chondrocyte

KW - Connective tissue growth factor

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Novel intracellular effects of human connective tissue growth factor expressed in Cos-7 cells

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Keywords

ASJC Scopus subject areas

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