Novel in vitro screening system for monoclonal antibodies using hypermutating chicken B cell library

Kagefumi Todo, Kenji Miyake, Masaki Magari, Naoki Kanayama, Hitoshi Ohmori

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Here, we report an in vitro screening system for monoclonal antibodies using a hypermutating chicken B cell line, DT40-SW. When switching on hypermutation, cultured DT40-SW cells constituted an antibody library, from which clones secreting antibodies to a test antigen were successfully isolated, and genetically stabilized by switching off the mutation machinery.

Original languageEnglish
Pages (from-to)478-481
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume102
Issue number5
DOIs
Publication statusPublished - Nov 1 2006

Keywords

  • chicken B cell line
  • hypermutation
  • monoclonal antibody

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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