Novel hydroxyapatite-polysaccharide composite microparticles for immunotherapy

Mitsuhiro Yoshida, Tomohiko Yoshioka, Toshiyuki Ikoma, Junzo Tanaka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Hydroxyapatite-chondroitin sulfate (HAp/ChS) composites were synthesized with calcium hydroxide suspension and phosphoric acid solution containing ChS through a precipitation method, and the microparticles were then fabricated by a spray dry method with the suspension of the composites. Bovine serum albumin (BSA) with negative charge or lysozyme (LYZ) with positive charge at pH7.0 was adsorbed onto the HAp/ChS microparticles. However, the HAp/ChS microparticles adsorbed LYZ more than the HAp microparticles compared with BSA due to the electrostatic interaction from negatively-charged sulfate or carboxyl group of ChS in the composites. The release property of BSA from the HAp/ChS microparticles was evaluated in Dulbecco's phosphate buffered saline (pH7.2). The HAp/ChS microparticles released quickly 100% of the adsorbed BSA, while HAp microparticles released 45% of BSA. These results indicated that incorporation of ChS in the microparticles controls the adsorption and release properties of protein due to the electrostatic interaction. The HAp/ChS microparticles therefore are a candidate of a carrier for drugs like vaccines.

Original languageEnglish
Title of host publicationMaterials Research Society Symposium Proceedings
PublisherMaterials Research Society
Volume1626
DOIs
Publication statusPublished - 2014
Externally publishedYes
Event2013 MRS Fall Meeting - Boston, MA, United States
Duration: Dec 1 2013Dec 6 2013

Other

Other2013 MRS Fall Meeting
CountryUnited States
CityBoston, MA
Period12/1/1312/6/13

Fingerprint

Chondroitin Sulfates
polysaccharides
microparticles
Durapatite
Polysaccharides
Hydroxyapatite
Bovine Serum Albumin
sulfates
composite materials
albumins
Composite materials
serums
Muramidase
Coulomb interactions
Suspensions
lysozyme
Enzymes
Calcium Hydroxide
Drug Carriers
Hydrated lime

Keywords

  • adsorption
  • ceramic
  • spray-drying

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

Yoshida, M., Yoshioka, T., Ikoma, T., & Tanaka, J. (2014). Novel hydroxyapatite-polysaccharide composite microparticles for immunotherapy. In Materials Research Society Symposium Proceedings (Vol. 1626). Materials Research Society. https://doi.org/10.1557/opl.2014.43

Novel hydroxyapatite-polysaccharide composite microparticles for immunotherapy. / Yoshida, Mitsuhiro; Yoshioka, Tomohiko; Ikoma, Toshiyuki; Tanaka, Junzo.

Materials Research Society Symposium Proceedings. Vol. 1626 Materials Research Society, 2014.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yoshida, M, Yoshioka, T, Ikoma, T & Tanaka, J 2014, Novel hydroxyapatite-polysaccharide composite microparticles for immunotherapy. in Materials Research Society Symposium Proceedings. vol. 1626, Materials Research Society, 2013 MRS Fall Meeting, Boston, MA, United States, 12/1/13. https://doi.org/10.1557/opl.2014.43
Yoshida M, Yoshioka T, Ikoma T, Tanaka J. Novel hydroxyapatite-polysaccharide composite microparticles for immunotherapy. In Materials Research Society Symposium Proceedings. Vol. 1626. Materials Research Society. 2014 https://doi.org/10.1557/opl.2014.43
Yoshida, Mitsuhiro ; Yoshioka, Tomohiko ; Ikoma, Toshiyuki ; Tanaka, Junzo. / Novel hydroxyapatite-polysaccharide composite microparticles for immunotherapy. Materials Research Society Symposium Proceedings. Vol. 1626 Materials Research Society, 2014.
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