Novel hydroxyapatite-polysaccharide composite microparticles for immunotherapy

Mitsuhiro Yoshida, Tomohiko Yoshioka, Toshiyuki Ikoma, Junzo Tanaka

Research output: Contribution to journalArticlepeer-review


Hydroxyapatite-chondroitin sulfate (HAp/ChS) composites were synthesized with calcium hydroxide suspension and phosphoric acid solution containing ChS through a precipitation method, and the microparticles were then fabricated by a spray dry method with the suspension of the composites. Bovine serum albumin (BSA) with negative charge or lysozyme (LYZ) with positive charge at pH7.0 was adsorbed onto the HAp/ChS microparticles. However, the HAp/ChS microparticles adsorbed LYZ more than the HAp microparticles compared with BSA due to the electrostatic interaction from negatively-charged sulfate or carboxyl group of ChS in the composites. The release property of BSA from the HAp/ChS microparticles was evaluated in Dulbecco's phosphate buffered saline (pH7.2). The HAp/ChS microparticles released quickly 100% of the adsorbed BSA, while HAp microparticles released 45% of BSA. These results indicated that incorporation of ChS in the microparticles controls the adsorption and release properties of protein due to the electrostatic interaction. The HAp/ChS microparticles therefore are a candidate of a carrier for drugs like vaccines.

Original languageEnglish
JournalPrehospital and Disaster Medicine
Issue number1
Publication statusPublished - Jan 7 2014
Externally publishedYes


  • adsorption
  • ceramic
  • spray-drying

ASJC Scopus subject areas

  • Emergency Medicine
  • Emergency


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