Abstract
We examined 24 C-type hepatitis specimens from Thailand and detected hepatitis C virus (HCV) RNA in all of them by RT-nested PCR for a portion of the HCV 5' non-coding (5' NC) region and a portion of the HCV core region. However, we failed to detect HCV RNA in 11 specimens by RT-nested PCR for a portion in the non-structural protein 5 (NS5) region that has been used commonly for HCV genotyping. We designed a new primer set for a separate portion of the NS5 region. Using this primer set, we succeeded in amplifying this portion in all 24 specimens. Two novel HCV genotypes, tentatively designated HCV-VII and HCV-VIII, were identified by sequencing these amplified regions. Our newly designed primers for RT-nested PCR may be useful for diagnosing infection as well as for genotyping unidentified HCV genomes.
| Original language | English |
|---|---|
| Pages (from-to) | 2323-2327 |
| Number of pages | 5 |
| Journal | Journal of General Virology |
| Volume | 76 |
| Issue number | 9 |
| Publication status | Published - 1995 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
- Virology
Cite this
Novel genotypes of hepatitis C virus in Thailand. / Sugiyama, K.; Kato, Nobuyuki; Nakazawa, T.; Yonemura, Y.; Phornphutkul, K.; Kunakorn, M.; Petchclai, B.; Shimotohno, K.
In: Journal of General Virology, Vol. 76, No. 9, 1995, p. 2323-2327.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Novel genotypes of hepatitis C virus in Thailand
AU - Sugiyama, K.
AU - Kato, Nobuyuki
AU - Nakazawa, T.
AU - Yonemura, Y.
AU - Phornphutkul, K.
AU - Kunakorn, M.
AU - Petchclai, B.
AU - Shimotohno, K.
PY - 1995
Y1 - 1995
N2 - We examined 24 C-type hepatitis specimens from Thailand and detected hepatitis C virus (HCV) RNA in all of them by RT-nested PCR for a portion of the HCV 5' non-coding (5' NC) region and a portion of the HCV core region. However, we failed to detect HCV RNA in 11 specimens by RT-nested PCR for a portion in the non-structural protein 5 (NS5) region that has been used commonly for HCV genotyping. We designed a new primer set for a separate portion of the NS5 region. Using this primer set, we succeeded in amplifying this portion in all 24 specimens. Two novel HCV genotypes, tentatively designated HCV-VII and HCV-VIII, were identified by sequencing these amplified regions. Our newly designed primers for RT-nested PCR may be useful for diagnosing infection as well as for genotyping unidentified HCV genomes.
AB - We examined 24 C-type hepatitis specimens from Thailand and detected hepatitis C virus (HCV) RNA in all of them by RT-nested PCR for a portion of the HCV 5' non-coding (5' NC) region and a portion of the HCV core region. However, we failed to detect HCV RNA in 11 specimens by RT-nested PCR for a portion in the non-structural protein 5 (NS5) region that has been used commonly for HCV genotyping. We designed a new primer set for a separate portion of the NS5 region. Using this primer set, we succeeded in amplifying this portion in all 24 specimens. Two novel HCV genotypes, tentatively designated HCV-VII and HCV-VIII, were identified by sequencing these amplified regions. Our newly designed primers for RT-nested PCR may be useful for diagnosing infection as well as for genotyping unidentified HCV genomes.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029164339&partnerID=8YFLogxK
M3 - Article
VL - 76
SP - 2323
EP - 2327
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 9
ER -