Novel genotypes of hepatitis C virus in Thailand

K. Sugiyama, Nobuyuki Kato, T. Nakazawa, Y. Yonemura, K. Phornphutkul, M. Kunakorn, B. Petchclai, K. Shimotohno

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We examined 24 C-type hepatitis specimens from Thailand and detected hepatitis C virus (HCV) RNA in all of them by RT-nested PCR for a portion of the HCV 5' non-coding (5' NC) region and a portion of the HCV core region. However, we failed to detect HCV RNA in 11 specimens by RT-nested PCR for a portion in the non-structural protein 5 (NS5) region that has been used commonly for HCV genotyping. We designed a new primer set for a separate portion of the NS5 region. Using this primer set, we succeeded in amplifying this portion in all 24 specimens. Two novel HCV genotypes, tentatively designated HCV-VII and HCV-VIII, were identified by sequencing these amplified regions. Our newly designed primers for RT-nested PCR may be useful for diagnosing infection as well as for genotyping unidentified HCV genomes.

Original languageEnglish
Pages (from-to)2323-2327
Number of pages5
JournalJournal of General Virology
Volume76
Issue number9
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Thailand
Hepacivirus
Genotype
Polymerase Chain Reaction
RNA
Hepatitis C
Proteins
Genome
Infection

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Sugiyama, K., Kato, N., Nakazawa, T., Yonemura, Y., Phornphutkul, K., Kunakorn, M., ... Shimotohno, K. (1995). Novel genotypes of hepatitis C virus in Thailand. Journal of General Virology, 76(9), 2323-2327.

Novel genotypes of hepatitis C virus in Thailand. / Sugiyama, K.; Kato, Nobuyuki; Nakazawa, T.; Yonemura, Y.; Phornphutkul, K.; Kunakorn, M.; Petchclai, B.; Shimotohno, K.

In: Journal of General Virology, Vol. 76, No. 9, 1995, p. 2323-2327.

Research output: Contribution to journalArticle

Sugiyama, K, Kato, N, Nakazawa, T, Yonemura, Y, Phornphutkul, K, Kunakorn, M, Petchclai, B & Shimotohno, K 1995, 'Novel genotypes of hepatitis C virus in Thailand', Journal of General Virology, vol. 76, no. 9, pp. 2323-2327.
Sugiyama K, Kato N, Nakazawa T, Yonemura Y, Phornphutkul K, Kunakorn M et al. Novel genotypes of hepatitis C virus in Thailand. Journal of General Virology. 1995;76(9):2323-2327.
Sugiyama, K. ; Kato, Nobuyuki ; Nakazawa, T. ; Yonemura, Y. ; Phornphutkul, K. ; Kunakorn, M. ; Petchclai, B. ; Shimotohno, K. / Novel genotypes of hepatitis C virus in Thailand. In: Journal of General Virology. 1995 ; Vol. 76, No. 9. pp. 2323-2327.
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