Novel genes involved in Ciona intestinalis embryogenesis

Characterization of gene knockdown embryos

Mayuko Hamada, Shuichi Wada, Kenji Kobayashi, Nori Satoh

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The sequenced genome of the urochordate ascidian Ciona intestinalis contains nearly 2,500 genes that have vertebrate homologues, but their functions are as yet unknown. To identify novel genes involved in early chordates embryogenesis, we previously screened 200 Ciona genes by knockdown experiments using specific morpholino oligonucleotides and found that suppression of the translation of 40 genes caused embryonic defects (Yamada et al. [2003] Development 130:6485-6495). We have since examined an additional 304 genes, that is, screening 504 genes overall, and a total of 111 genes showed morphological defects when gene function was suppressed. We further examined the role of these genes in the differentiation of six major tissues of the embryo: endoderm, muscle, epidermis, neural tissue, mesenchyme, and notochord. Based on the similarity of phenotypes of gene knockdown embryos, genes were categorized into several groups, with the suggestion that the genes within a given group are involved in similar developmental processes. For example, five were shown to be novel genes that are likely involved in β-catenin-mediated endoderm formation. The type of large-scale screening used is, therefore, a powerful approach to identify novel genes with significant developmental functions, the details of which will be determined in future studies.

Original languageEnglish
Pages (from-to)1820-1831
Number of pages12
JournalDevelopmental Dynamics
Volume236
Issue number7
DOIs
Publication statusPublished - Jul 2007
Externally publishedYes

Fingerprint

Ciona intestinalis
Gene Knockdown Techniques
Embryonic Development
Embryonic Structures
Genes
Urochordata
Endoderm
Chordata
Notochord
Catenins
Morpholinos
Mesoderm
Epidermis

Keywords

  • Ciona intestinalis
  • Embryogenesis
  • Functional screening
  • Morpholino
  • Novel genes
  • Tissue differentiation

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Novel genes involved in Ciona intestinalis embryogenesis : Characterization of gene knockdown embryos. / Hamada, Mayuko; Wada, Shuichi; Kobayashi, Kenji; Satoh, Nori.

In: Developmental Dynamics, Vol. 236, No. 7, 07.2007, p. 1820-1831.

Research output: Contribution to journalArticle

Hamada, Mayuko ; Wada, Shuichi ; Kobayashi, Kenji ; Satoh, Nori. / Novel genes involved in Ciona intestinalis embryogenesis : Characterization of gene knockdown embryos. In: Developmental Dynamics. 2007 ; Vol. 236, No. 7. pp. 1820-1831.
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