Novel FNR homologues identified in four representative oral facultative anaerobes

Capnocytophaga ochracea, Capnocytophaga sputigena, Haemophilus aphrophilus, and Actinobacillus actinomycetemcomitans

Takako Hattori, Kojiro Takahashi, Tohru Nakanishi, Hiroyuki Ohta, Kazuhiro Fukui, Shigehiko Taniguchi, Masaharu Takigawa

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Based upon DNA sequence data and positive immunochemical reactivity of expressed protein, novel homologues of the FNR family were identified in four representative oral facultative anaerobes: Capnocytophaga ochracea, Capnocytophaga sputigena, Haemophilus aphrophilus, and Actinobacillus actinomycetemcomitans. The similarity to E. coli FNR and to HlyX (itself 71% similar to E. coli FNR, while regulating expression of hemolysin operon in Actinobacillus pleuropneumoniae) was estimated from the deduced partial amino acid sequence to be, in the above order of tested species, 98, 98, 86, and 85%, and 75, 75, 88, and 88%, respectively. The phylogenetic relatedness indicates a rather closer link of HlyX to the FNR homologues from both pathogens, H. aphrophilus and A. actinomycetemcomitans. The possibility that the A. actinomycetemcomitans FNR homologue functions as a redox-sensing transcriptional factor to regulate, in addition to anaerobic respiration, microaerobic expression of the leukotoxin operon (ltx gene) is suggested.

Original languageEnglish
Pages (from-to)213-220
Number of pages8
JournalFEMS Microbiology Letters
Volume137
Issue number2-3
DOIs
Publication statusPublished - Apr 1 1996

Fingerprint

Aggregatibacter aphrophilus
Capnocytophaga
Aggregatibacter actinomycetemcomitans
Operon
Actinobacillus pleuropneumoniae
Escherichia coli
Hemolysin Proteins
Oxidation-Reduction
Amino Acid Sequence
Respiration
Genes

Keywords

  • Actinobacillus
  • anaero-box
  • Capnocytophaga
  • facultative anaerobe
  • FNR
  • Haemophilus
  • HlyX
  • phylogenetic relatedness
  • transcriptional regulator

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Novel FNR homologues identified in four representative oral facultative anaerobes : Capnocytophaga ochracea, Capnocytophaga sputigena, Haemophilus aphrophilus, and Actinobacillus actinomycetemcomitans. / Hattori, Takako; Takahashi, Kojiro; Nakanishi, Tohru; Ohta, Hiroyuki; Fukui, Kazuhiro; Taniguchi, Shigehiko; Takigawa, Masaharu.

In: FEMS Microbiology Letters, Vol. 137, No. 2-3, 01.04.1996, p. 213-220.

Research output: Contribution to journalArticle

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