Novel features of eukaryotic photosystem II revealed by its crystal structure analysis from a red alga

Hideo Ago, Hideyuki Adachi, Yasufumi Umena, Takayoshi Tashiro, Keisuke Kawakami, Nobuo Kamiya Lirong Tian, Guangye Han, Tingyun Kuang, Zheyi Liu, Fangjun Wang, Hanfa Zou, Isao Enami, Masashi Miyano, Jian-Ren Shen

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Abstract

Photosystem II (PSII) catalyzes light-induced water splitting, leading to the evolution of molecular oxygen indispensible for life on the earth. The crystal structure of PSII from cyanobacteria has been solved at an atomic level, but the structure of eukaryotic PSII has not been analyzed. Because eukaryotic PSII possesses additional subunits not found in cyanobacterial PSII, it is important to solve the structure of eukaryotic PSII to elucidate their detailed functions, as well as evolutionary relationships. Here we report the structure of PSII from a red alga Cyanidium caldarium at 2.76 Å resolution, which revealed the structure and interaction sites of PsbQ′, a unique, fourth extrinsic protein required for stabilizing the oxygen-evolving complex in the lumenal surface of PSII. The PsbQ′ subunit was found to be located underneath CP43 in the vicinity of PsbV, and its structure is characterized by a bundle of four up-down helices arranged in a similar way to those of cyanobacterial and higher plant PsbQ, although helices I and II of PsbQ′ were kinked relative to its higher plant counterpart because of its interactions with CP43. Furthermore, two novel transmembrane helices were found in the red algal PSII that are not present in cyanobacterial PSII; one of these helices may correspond to PsbW found only in eukaryotic PSII. The present results represent the first crystal structure of PSII from eukaryotic oxygenic organisms, which were discussed in comparison with the structure of cyanobacterial PSII.

Original languageEnglish
Pages (from-to)5676-5687
Number of pages12
JournalJournal of Biological Chemistry
Volume291
Issue number11
DOIs
Publication statusPublished - Mar 11 2016

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Rhodophyta
Photosystem II Protein Complex
Algae
Crystal structure
Oxygen
Molecular Evolution
Molecular oxygen
Cyanobacteria

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Novel features of eukaryotic photosystem II revealed by its crystal structure analysis from a red alga. / Ago, Hideo; Adachi, Hideyuki; Umena, Yasufumi; Tashiro, Takayoshi; Kawakami, Keisuke; Tian, Nobuo Kamiya Lirong; Han, Guangye; Kuang, Tingyun; Liu, Zheyi; Wang, Fangjun; Zou, Hanfa; Enami, Isao; Miyano, Masashi; Shen, Jian-Ren.

In: Journal of Biological Chemistry, Vol. 291, No. 11, 11.03.2016, p. 5676-5687.

Research output: Contribution to journalArticle

Ago, H, Adachi, H, Umena, Y, Tashiro, T, Kawakami, K, Tian, NKL, Han, G, Kuang, T, Liu, Z, Wang, F, Zou, H, Enami, I, Miyano, M & Shen, J-R 2016, 'Novel features of eukaryotic photosystem II revealed by its crystal structure analysis from a red alga', Journal of Biological Chemistry, vol. 291, no. 11, pp. 5676-5687. https://doi.org/10.1074/jbc.M115.711689
Ago, Hideo ; Adachi, Hideyuki ; Umena, Yasufumi ; Tashiro, Takayoshi ; Kawakami, Keisuke ; Tian, Nobuo Kamiya Lirong ; Han, Guangye ; Kuang, Tingyun ; Liu, Zheyi ; Wang, Fangjun ; Zou, Hanfa ; Enami, Isao ; Miyano, Masashi ; Shen, Jian-Ren. / Novel features of eukaryotic photosystem II revealed by its crystal structure analysis from a red alga. In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 11. pp. 5676-5687.
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AU - Han, Guangye

AU - Kuang, Tingyun

AU - Liu, Zheyi

AU - Wang, Fangjun

AU - Zou, Hanfa

AU - Enami, Isao

AU - Miyano, Masashi

AU - Shen, Jian-Ren

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