Novel Enzyme-Linked Immunosorbent Assay Systems for the Quantitative Analysis of Connective Tissue Growth Factor (CTGF/Hcs24/CCN2): Detection of HTLV-I Tax-Induced CTGF from a Human Carcinoma Cell Line

Harumi Kawaki, Satoshi Kubota, Masanao Minato, Norifumi Moritani, Takako Hattori, Hiroshi Hanagata, Mamoru Kubota, Akira Miyauchi, Tohru Nakanishi, Masaharu Takigawa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24/CCN2) is known as a multifunctional growth factor. It stimulates proliferation, migration, and extracellular matrix production of mesenchymal cells, and is highly expressed in hypertrophic chondrocytes. In this study, we constructed useful ELISA systems for the analysis of CTGF and its modular fragments. For this objective we prepared four different antihuman CTGF monoclonal antibodies. One, specific for the VWC module, was utilized as the detecting antibody, and the other three, recognizing CT, IGFBP, and VWC modules, respectively, were employed as capture antibodies. Then we established three novel quantitative analysis systems for CTGF. The first system recognizing CT and VWC modules was useful to measure full-length CTGF with improved sensitivity. Utilizing this system, we found significant enhancement of CTGF production from a human carcinoma cell line transduced by HTLV-I tax gene, where the finding indicates the possible involvement of Tax in carcinogenesis. The second system, seeing IGFBP and VWC modules, could quantify not only CTGF, but also may be useful to analyze processed N-terminal fragments. The third system, utilizing capture and detection antibodies against the VWC module, was able to quantify the VWC module only, while it did not recognize full-length CTGF. Since CTGF is actually processed into subfragments, and functional assignment of each module is of interest, these systems are expected to contribute to the progress of CTGF investigations.

Original languageEnglish
Pages (from-to)641-648
Number of pages8
JournalDNA and Cell Biology
Volume22
Issue number10
DOIs
Publication statusPublished - Oct 2003

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Connective Tissue Growth Factor
Human T-lymphotropic virus 1
Insulin-Like Growth Factor Binding Proteins
Enzyme-Linked Immunosorbent Assay
Chondrocytes
Carcinoma
Cell Line
Antibodies
pX Genes
Systems Analysis
Extracellular Matrix
Intercellular Signaling Peptides and Proteins
Carcinogenesis
Monoclonal Antibodies
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Novel Enzyme-Linked Immunosorbent Assay Systems for the Quantitative Analysis of Connective Tissue Growth Factor (CTGF/Hcs24/CCN2) : Detection of HTLV-I Tax-Induced CTGF from a Human Carcinoma Cell Line. / Kawaki, Harumi; Kubota, Satoshi; Minato, Masanao; Moritani, Norifumi; Hattori, Takako; Hanagata, Hiroshi; Kubota, Mamoru; Miyauchi, Akira; Nakanishi, Tohru; Takigawa, Masaharu.

In: DNA and Cell Biology, Vol. 22, No. 10, 10.2003, p. 641-648.

Research output: Contribution to journalArticle

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