Novel, diverse RNA viruses from Mediterranean isolates of the phytopathogenic fungus, Rosellinia necatrix

insights into evolutionary biology of fungal viruses

Juan Manuel Arjona-Lopez, Paul Telengech, Atif Jamal, Sakae Hisano, Hideki Kondo, Mery Dafny Yelin, Isabel Arjona-Girona, Satoko Kanematsu, Carlos José Lopez-Herrera, Nobuhiro Suzuki

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

To reveal mycovirus diversity, we conducted a search of as-yet-unexplored Mediterranean isolates of the phytopathogenic ascomycete Rosellinia necatrix for virus infections. Of seventy-nine, eleven fungal isolates tested RNA virus-positive, with many showing coinfections, indicating a virus incidence of 14%, which is slightly lower than that (approximately 20%) previously reported for extensive surveys of over 1000 Japanese R. necatrix isolates. All viral sequences were fully or partially characterized by Sanger and next-generation sequencing. These sequences appear to represent isolates of various new species spanning at least 6 established or previously proposed families such as Partiti-, Hypo-, Megabirna-, Yado-kari-, Fusagra- and Fusarividae, as well as a newly proposed family, Megatotiviridae. This observation greatly expands the diversity of R. necatrix viruses, because no hypo-, fusagra- or megatotiviruses were previously reported from R. necatrix. The sequence analyses showed a rare horizontal gene transfer event of the 2A-like protease domain between a dsRNA (phlegivirus) and a positive-sense, single-stranded RNA virus (hypovirus). Moreover, many of the newly detected viruses showed the closest relation to viruses reported from fungi other than R. necatrix, such as Fusarium spp., which are sympatric to R. necatrix. These combined results imply horizontal virus transfer between these soil-inhabitant fungi.

Original languageEnglish
Pages (from-to)1464-1483
Number of pages20
JournalEnvironmental Microbiology
Volume20
Issue number4
DOIs
Publication statusPublished - Apr 1 2018

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mycoviruses
Rosellinia necatrix
RNA Viruses
evolutionary biology
plant pathogenic fungi
RNA
virus
Fungi
fungus
Viruses
Biological Sciences
viruses
positive sense, single-stranded RNA viruses
Hypovirus
Horizontal Gene Transfer
Ascomycota
Fusarium
Virus Diseases
Coinfection
soil fungi

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Novel, diverse RNA viruses from Mediterranean isolates of the phytopathogenic fungus, Rosellinia necatrix : insights into evolutionary biology of fungal viruses. / Arjona-Lopez, Juan Manuel; Telengech, Paul; Jamal, Atif; Hisano, Sakae; Kondo, Hideki; Yelin, Mery Dafny; Arjona-Girona, Isabel; Kanematsu, Satoko; Lopez-Herrera, Carlos José; Suzuki, Nobuhiro.

In: Environmental Microbiology, Vol. 20, No. 4, 01.04.2018, p. 1464-1483.

Research output: Contribution to journalArticle

Arjona-Lopez, Juan Manuel ; Telengech, Paul ; Jamal, Atif ; Hisano, Sakae ; Kondo, Hideki ; Yelin, Mery Dafny ; Arjona-Girona, Isabel ; Kanematsu, Satoko ; Lopez-Herrera, Carlos José ; Suzuki, Nobuhiro. / Novel, diverse RNA viruses from Mediterranean isolates of the phytopathogenic fungus, Rosellinia necatrix : insights into evolutionary biology of fungal viruses. In: Environmental Microbiology. 2018 ; Vol. 20, No. 4. pp. 1464-1483.
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