Novel CD200 homologues iSEC1 and iSEC2 are gastrointestinal secretory cell-specific ligands of inhibitory receptor CD200R

Toshiyuki Kojima, Kiichiro Tsuchiya, Shinji Ikemizu, Soichiro Yoshikawa, Yoshinori Yamanishi, Mamoru Watanabe, Hajime Karasuyama

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

CD200R is an inhibitory receptor expressed on myeloid cells and some lymphoid cells, and plays important roles in negatively regulating immune responses. CD200 is the only known ligand of CD200R and broadly distributed in a variety of cell types. Here we identified novel CD200 homologues, designated iSEC1 and iSEC2, that are expressed exclusively by secretory cell lineages in the gastrointestinal epithelium while authentic CD200 is expressed by none of epithelial cells including secretory cells. Both iSEC1 and iSEC2 could bind to CD200R but not other members of the CD200R family. Notably, CD200R expression was confined to intraepithelial lymphocytes (IELs) among cells in the gastrointestinal epithelium. Binding of iSEC1 to CD200R on IELs resulted in the suppression of cytokine production and cytolytic activity by activated IELs. Thus, iSEC1 is a previously unappreciated CD200R ligand with restricted expression in gastrointestinal secretory cells and may negatively regulate mucosal immune responses.

Original languageEnglish
Article number36457
JournalScientific reports
Volume6
DOIs
Publication statusPublished - Nov 7 2016
Externally publishedYes

Fingerprint

Lymphocytes
Ligands
Epithelium
Mucosal Immunity
Cell Lineage
Myeloid Cells
Epithelial Cells
Cytokines

ASJC Scopus subject areas

  • General

Cite this

Novel CD200 homologues iSEC1 and iSEC2 are gastrointestinal secretory cell-specific ligands of inhibitory receptor CD200R. / Kojima, Toshiyuki; Tsuchiya, Kiichiro; Ikemizu, Shinji; Yoshikawa, Soichiro; Yamanishi, Yoshinori; Watanabe, Mamoru; Karasuyama, Hajime.

In: Scientific reports, Vol. 6, 36457, 07.11.2016.

Research output: Contribution to journalArticle

Kojima, Toshiyuki ; Tsuchiya, Kiichiro ; Ikemizu, Shinji ; Yoshikawa, Soichiro ; Yamanishi, Yoshinori ; Watanabe, Mamoru ; Karasuyama, Hajime. / Novel CD200 homologues iSEC1 and iSEC2 are gastrointestinal secretory cell-specific ligands of inhibitory receptor CD200R. In: Scientific reports. 2016 ; Vol. 6.
@article{1ddd5f52b06546e482214fa2fd18c77b,
title = "Novel CD200 homologues iSEC1 and iSEC2 are gastrointestinal secretory cell-specific ligands of inhibitory receptor CD200R",
abstract = "CD200R is an inhibitory receptor expressed on myeloid cells and some lymphoid cells, and plays important roles in negatively regulating immune responses. CD200 is the only known ligand of CD200R and broadly distributed in a variety of cell types. Here we identified novel CD200 homologues, designated iSEC1 and iSEC2, that are expressed exclusively by secretory cell lineages in the gastrointestinal epithelium while authentic CD200 is expressed by none of epithelial cells including secretory cells. Both iSEC1 and iSEC2 could bind to CD200R but not other members of the CD200R family. Notably, CD200R expression was confined to intraepithelial lymphocytes (IELs) among cells in the gastrointestinal epithelium. Binding of iSEC1 to CD200R on IELs resulted in the suppression of cytokine production and cytolytic activity by activated IELs. Thus, iSEC1 is a previously unappreciated CD200R ligand with restricted expression in gastrointestinal secretory cells and may negatively regulate mucosal immune responses.",
author = "Toshiyuki Kojima and Kiichiro Tsuchiya and Shinji Ikemizu and Soichiro Yoshikawa and Yoshinori Yamanishi and Mamoru Watanabe and Hajime Karasuyama",
year = "2016",
month = "11",
day = "7",
doi = "10.1038/srep36457",
language = "English",
volume = "6",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Novel CD200 homologues iSEC1 and iSEC2 are gastrointestinal secretory cell-specific ligands of inhibitory receptor CD200R

AU - Kojima, Toshiyuki

AU - Tsuchiya, Kiichiro

AU - Ikemizu, Shinji

AU - Yoshikawa, Soichiro

AU - Yamanishi, Yoshinori

AU - Watanabe, Mamoru

AU - Karasuyama, Hajime

PY - 2016/11/7

Y1 - 2016/11/7

N2 - CD200R is an inhibitory receptor expressed on myeloid cells and some lymphoid cells, and plays important roles in negatively regulating immune responses. CD200 is the only known ligand of CD200R and broadly distributed in a variety of cell types. Here we identified novel CD200 homologues, designated iSEC1 and iSEC2, that are expressed exclusively by secretory cell lineages in the gastrointestinal epithelium while authentic CD200 is expressed by none of epithelial cells including secretory cells. Both iSEC1 and iSEC2 could bind to CD200R but not other members of the CD200R family. Notably, CD200R expression was confined to intraepithelial lymphocytes (IELs) among cells in the gastrointestinal epithelium. Binding of iSEC1 to CD200R on IELs resulted in the suppression of cytokine production and cytolytic activity by activated IELs. Thus, iSEC1 is a previously unappreciated CD200R ligand with restricted expression in gastrointestinal secretory cells and may negatively regulate mucosal immune responses.

AB - CD200R is an inhibitory receptor expressed on myeloid cells and some lymphoid cells, and plays important roles in negatively regulating immune responses. CD200 is the only known ligand of CD200R and broadly distributed in a variety of cell types. Here we identified novel CD200 homologues, designated iSEC1 and iSEC2, that are expressed exclusively by secretory cell lineages in the gastrointestinal epithelium while authentic CD200 is expressed by none of epithelial cells including secretory cells. Both iSEC1 and iSEC2 could bind to CD200R but not other members of the CD200R family. Notably, CD200R expression was confined to intraepithelial lymphocytes (IELs) among cells in the gastrointestinal epithelium. Binding of iSEC1 to CD200R on IELs resulted in the suppression of cytokine production and cytolytic activity by activated IELs. Thus, iSEC1 is a previously unappreciated CD200R ligand with restricted expression in gastrointestinal secretory cells and may negatively regulate mucosal immune responses.

UR - http://www.scopus.com/inward/record.url?scp=84994644870&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994644870&partnerID=8YFLogxK

U2 - 10.1038/srep36457

DO - 10.1038/srep36457

M3 - Article

C2 - 27819346

AN - SCOPUS:84994644870

VL - 6

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 36457

ER -