Novel assay system for acidic Peptide:N-glycanase (aPNGase) activity in crude plant extract

Ryota Uemura, Mikako Ogura, Chihiro Matsumaru, Tsuyoshi Akiyama, Megumi Maeda, Yoshinobu Kimura

Research output: Contribution to journalArticle

Abstract

Acidic peptide:N-glycanase (aPNGase) plays a pivotal role in plant glycoprotein turnover. For the construction of aPNGase-knockout or -overexpressing plants, a new method to detect the activity in crude plant extracts is required because endogenous peptidases present in the extract hamper enzyme assays using fluorescence-labeled N-glycopeptides as a substrate. In this study, we developed a new method for measuring aPNGase activity in crude extracts from plant materials.

Original languageEnglish
Pages (from-to)1172-1175
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume82
Issue number7
DOIs
Publication statusPublished - 2018

Keywords

  • Acidic Pngase
  • Affinity chromatography
  • Enzyme assay
  • Free N-glycans
  • Transgenic plant

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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