Novel assay system for acidic Peptide: N-glycanase (aPNGase) activity in crude plant extract

Ryota Uemura, Mikako Ogura, Chihiro Matsumaru, Tsuyoshi Akiyama, Megumi Maeda, Yoshinobu Kimura

Research output: Contribution to journalArticle

Abstract

Acidic peptide:N-glycanase (aPNGase) plays a pivotal role in plant glycoprotein turnover. For the construction of aPNGase-knockout or -overexpressing plants, a new method to detect the activity in crude plant extracts is required because endogenous peptidases present in the extract hamper enzyme assays using fluorescence-labeled N-glycopeptides as a substrate. In this study, we developed a new method for measuring aPNGase activity in crude extracts from plant materials.

Original languageEnglish
Pages (from-to)1172-1175
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume82
Issue number7
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Plant Extracts
Complex Mixtures
Assays
Glycopeptides
Enzyme Assays
Glycoproteins
Peptide Hydrolases
Fluorescence
Substrates
Enzymes

Keywords

  • Acidic Pngase
  • Affinity chromatography
  • Enzyme assay
  • Free N-glycans
  • Transgenic plant

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Novel assay system for acidic Peptide : N-glycanase (aPNGase) activity in crude plant extract. / Uemura, Ryota; Ogura, Mikako; Matsumaru, Chihiro; Akiyama, Tsuyoshi; Maeda, Megumi; Kimura, Yoshinobu.

In: Bioscience, Biotechnology and Biochemistry, Vol. 82, No. 7, 01.01.2018, p. 1172-1175.

Research output: Contribution to journalArticle

Uemura, Ryota ; Ogura, Mikako ; Matsumaru, Chihiro ; Akiyama, Tsuyoshi ; Maeda, Megumi ; Kimura, Yoshinobu. / Novel assay system for acidic Peptide : N-glycanase (aPNGase) activity in crude plant extract. In: Bioscience, Biotechnology and Biochemistry. 2018 ; Vol. 82, No. 7. pp. 1172-1175.
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