Novel antimutagenic factors derived from the edible mushroom Agrocybe cylindracea

Kentaro Taira, Yukiko Miyashita, Keinosuke Okamoto, Sakae Arimoto, Eizo Takahashi, Tomoe Negishi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Studies have shown that certain foods contain compounds with antigenotoxic activities. Here, we ask if dried powders and/or extracts from three edible mushrooms, Agrocybe cylindracea, Lentinula edodes and Pleurotus ostreatus, have a mitigating effect on genotoxicity. We used two in vivo assays: the Drosophila DNA repair test and the Drosophila wing spot test (also known as SMART) which measures somatic mutation and recombination. Eight carcinogens were tested with the mushroom powders: 2-AAF, aflatoxin B1, DMBA, IQ, MeIQx, MNU NDMA, and 4NQO. We found that A. cylindracea and P. ostreatus powders can suppress DNA damage induced by each of the mutagens we tested. In contrast, L. edodes has an inhibitory effect on DNA damage induced by only a sub-set of mutagens, namely aflatoxin B1, NDMA, MNU and 4NQO. In addition, A. cylindracea extracts were able to suppress somatic cell mutation induced by aflatoxin B1, MMC, MNU, NDMA, NMOR and 4NQO. These results suggest that Agrocybe genus mushrooms contain factors with antigenotoxic activity, including anti-recombinogenic activity. Furthermore, the antigenotoxic activity of A. cylindracea powder can be extracted in water but not in ethyl acetate or methanol, and is sensitive to heat treatment. The data suggest that there is a novel antigenotoxic factor(s) in A. cylindracea, possibly in the form of a peptide or protein.

Original languageEnglish
Pages (from-to)115-123
Number of pages9
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume586
Issue number2
DOIs
Publication statusPublished - Oct 3 2005

Fingerprint

Agrocybe
Agaricales
Powders
Aflatoxin B1
Shiitake Mushrooms
Pleurotus
Mutagens
DNA Damage
Drosophila
2-Acetylaminofluorene
9,10-Dimethyl-1,2-benzanthracene
Mutation
DNA Repair
Carcinogens
Genetic Recombination
Methanol
Hot Temperature
Food
Peptides
Water

Keywords

  • Agrocybe
  • Ames test
  • Antigenotoxicity
  • Chemoprevention
  • Drosophila SMART
  • Mushroom

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Genetics

Cite this

Novel antimutagenic factors derived from the edible mushroom Agrocybe cylindracea. / Taira, Kentaro; Miyashita, Yukiko; Okamoto, Keinosuke; Arimoto, Sakae; Takahashi, Eizo; Negishi, Tomoe.

In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 586, No. 2, 03.10.2005, p. 115-123.

Research output: Contribution to journalArticle

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