Notch-, Wingless-, and Dpp-mediated signaling pathways are required for functional specification of Drosophila midgut cells

Ryushin Tanaka, Yoshikazu Takase, Masamitsu Kanachi, Rie Enomoto-Katayama, Tetsuya Shirai, Hideki Nakagoshi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The mechanisms for cell fate determination have been extensively studied whereas little is known about the mechanism through which functional specificity is established. In the Drosophila midgut, copper cells provide an excellent model system to examine this mechanism. Copper is an essential element for the activity of a number of physiologically important enzymes including Cu/Zn-superoxide dismutase, cytochrome c oxidase, and dopamine-β-hydroxylase. Drosophila copper cells are involved in two distinct functions, i.e., copper absorption and acid secretion, which are visualized as a fluorescent signal and a color change of a pH indicator dye, respectively. Here we show that the absorptive function is established through two independent pathways, the Notch signaling pathway in adjacent interstitial cells and the Wingless signaling pathway in copper cells. Furthermore, the other function, acid secretion, is regulated through the Decapentaplegic and Wingless signaling pathways in interstitial cells. Our results clearly indicate that normal morphological development is insufficient for functional maturation, and that subsequent functional specification is achieved through several independent pathways. These results provide valuable insights into the molecular mechanism underlying functional specification.

Original languageEnglish
Pages (from-to)53-61
Number of pages9
JournalDevelopmental Biology
Volume304
Issue number1
DOIs
Publication statusPublished - Apr 1 2007

Fingerprint

Drosophila
Copper
Acids
Electron Transport Complex IV
Mixed Function Oxygenases
Dopamine
Coloring Agents
Color
Enzymes

Keywords

  • Copper
  • Dpp
  • Drosophila
  • dve
  • Gut
  • Notch
  • Proton
  • Wingless

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Notch-, Wingless-, and Dpp-mediated signaling pathways are required for functional specification of Drosophila midgut cells. / Tanaka, Ryushin; Takase, Yoshikazu; Kanachi, Masamitsu; Enomoto-Katayama, Rie; Shirai, Tetsuya; Nakagoshi, Hideki.

In: Developmental Biology, Vol. 304, No. 1, 01.04.2007, p. 53-61.

Research output: Contribution to journalArticle

Tanaka, Ryushin ; Takase, Yoshikazu ; Kanachi, Masamitsu ; Enomoto-Katayama, Rie ; Shirai, Tetsuya ; Nakagoshi, Hideki. / Notch-, Wingless-, and Dpp-mediated signaling pathways are required for functional specification of Drosophila midgut cells. In: Developmental Biology. 2007 ; Vol. 304, No. 1. pp. 53-61.
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