Notch as a possible cell differentiation factor in pleomorphic adenomas

Keisuke Takamine, Yukiko Ueda, Keisuke Nakano, Takanaga Ochiai, Yoshihiko Sugita, Katsutoshi Kubo, Hatsuhiko Maeda, Hiromasa Hasegawa, Toshiyuki Kawakami

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Abstract

The expression of Notch in 30 cases of pleomorphic adenoma was examined by immunohisto-chemistry. Comparing the results of our study with previous literatures, from the partial CK7 expression and substantial Notch expression in ductal epithelial cells as well as the Notch expression in solid tumor nests, it can be inferred that Notch is involved in cell differentiation. CK13 expression was observed in cells undergoing squamous metaplasia and Notch expression was seen in the nucleus of basal and squamous cells. The intense Notch expression in basal cells and weak expression in squamous cells suggests that Notch is involved in the differentiation from basal to squamous cell. Moreover, the loss of nuclear expression on the inner layer would signify that differentiation is about to end or has been terminated. Notch was expressed in the cytoplasm of cartilage cells and in the cell membrane of mucous cells but not in the nucleus indicating that differentiation has been concluded. Notch involvement is suspected in cell differentiation in areas showing ductal structures and squamous metaplasia. In summary, Notch is involved in cell differentiation of ductal cells in PA. Nuclear expression was shown in tumor cells in solid nests and surrounding structures. Moreover, Notch is expressed by basal cells undergoing squamous metaplasia suggesting the participation of Notch in cell differentiation in PA.

Original languageEnglish
Pages (from-to)759-763
Number of pages5
JournalInternational Journal of Medical Sciences
Volume12
Issue number10
DOIs
Publication statusPublished - Sep 5 2015

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Pleomorphic Adenoma
Cell Differentiation
Epithelial Cells
Metaplasia
Basal Ganglia
Cartilage
Neoplasms
Cytoplasm
Cell Membrane

Keywords

  • Cell differentiation
  • Immunohistochemistry
  • Notch1
  • Pleomorphic adenoma

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Notch as a possible cell differentiation factor in pleomorphic adenomas. / Takamine, Keisuke; Ueda, Yukiko; Nakano, Keisuke; Ochiai, Takanaga; Sugita, Yoshihiko; Kubo, Katsutoshi; Maeda, Hatsuhiko; Hasegawa, Hiromasa; Kawakami, Toshiyuki.

In: International Journal of Medical Sciences, Vol. 12, No. 10, 05.09.2015, p. 759-763.

Research output: Contribution to journalArticle

Takamine, K, Ueda, Y, Nakano, K, Ochiai, T, Sugita, Y, Kubo, K, Maeda, H, Hasegawa, H & Kawakami, T 2015, 'Notch as a possible cell differentiation factor in pleomorphic adenomas', International Journal of Medical Sciences, vol. 12, no. 10, pp. 759-763. https://doi.org/10.7150/ijms.12882
Takamine, Keisuke ; Ueda, Yukiko ; Nakano, Keisuke ; Ochiai, Takanaga ; Sugita, Yoshihiko ; Kubo, Katsutoshi ; Maeda, Hatsuhiko ; Hasegawa, Hiromasa ; Kawakami, Toshiyuki. / Notch as a possible cell differentiation factor in pleomorphic adenomas. In: International Journal of Medical Sciences. 2015 ; Vol. 12, No. 10. pp. 759-763.
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