Norspermine substitutes for thermospermine in the control of stem elongation in Arabidopsis thaliana

Jun Ichi Kakehi, Yoshitaka Kuwashiro, Hiroyasu Motose, Kazuei Igarashi, Taku Takahashi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Thermospermine is a structural isomer of spermine and is required for stem elongation in Arabidopsis thaliana. We noted the C3C3 arrangement of carbon chains in thermospermine (C3C3C4), which is not present in spermine (C3C4C3), and examined if it is functionally replaced with norspermine (C3C3C3) or not. Exogenous application of norspermine to acl5, a mutant defective in the synthesis of thermospermine, partially suppressed its dwarf phenotype, and down-regulated the level of the acl5 transcript which is much higher than that of the ACL5 transcript in the wild type. Furthermore, in the Zinnia culture, differentiation of mesophyll cells into tracheary elements was blocked by thermospermine and norspermine but not by spermine. Our results indicate that norspermine can functionally substitute for thermospermine.

Original languageEnglish
Pages (from-to)3042-3046
Number of pages5
JournalFEBS Letters
Volume584
Issue number14
DOIs
Publication statusPublished - Jul 1 2010

Keywords

  • Arabidopsis
  • Norspermine
  • Polyamine
  • Stem elongation
  • Thermospermine
  • Xylem

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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