Normothermic extracorporeal perfusion of isolated porcine liver after warm ischaemia: A preliminary report

Rinaldo Bellomo, Satoshi Suzuki, Bruno Marino, Graeme K. Starkey, Brenton Chambers, Michael A. Fink, Bao Zhong Wang, Shane Houston, Glenn Eastwood, Paolo Calzavacca, Neil Glassford, Alison Skene, Daryl A. Jones, Robert Jones

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Liver transplantation is a major life-saving procedure, and donation after cardiac death (DCD) has increased the pool of potential liver donors. However, DCD livers are at increased risk of primary graft dysfunction and biliary tract ischaemia. Normothermic extracorporeal liver perfusion (NELP) may increase the ability to protect, evaluate and, in future, transplant DCD livers. We conducted proof-of-concept experiments using a DCD model in the pig to assess the short-term (4 hours) feasibility and functional efficacy of NELP. Using extracorporeal membrane oxygenation, parenteral nutrition, separate hepatic artery and portal vein perfusion, and physiological perfusion pressures, we achieved NELP and evidence of function (bile production, paracetamol removal, maintenance of normal ammonia and lactate levels) for 4 hours in pig livers subjected to 15 and 30 minutes of cardiac arrest before explantation. Our experiments justify further investigations of the feasibility and efficacy of human DCD liver preservation by ex-vivo perfusion.

Original languageEnglish
Pages (from-to)173-176
Number of pages4
JournalCritical Care and Resuscitation
Volume14
Issue number3
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Warm Ischemia
Swine
Perfusion
Liver
Primary Graft Dysfunction
Extracorporeal Membrane Oxygenation
Hepatic Veins
Hepatic Artery
Parenteral Nutrition
Biliary Tract
Acetaminophen
Portal Vein
Heart Arrest
Ammonia
Bile
Liver Transplantation
Lactic Acid
Ischemia
Maintenance
Transplants

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine
  • Anesthesiology and Pain Medicine

Cite this

Bellomo, R., Suzuki, S., Marino, B., Starkey, G. K., Chambers, B., Fink, M. A., ... Jones, R. (2012). Normothermic extracorporeal perfusion of isolated porcine liver after warm ischaemia: A preliminary report. Critical Care and Resuscitation, 14(3), 173-176.

Normothermic extracorporeal perfusion of isolated porcine liver after warm ischaemia : A preliminary report. / Bellomo, Rinaldo; Suzuki, Satoshi; Marino, Bruno; Starkey, Graeme K.; Chambers, Brenton; Fink, Michael A.; Wang, Bao Zhong; Houston, Shane; Eastwood, Glenn; Calzavacca, Paolo; Glassford, Neil; Skene, Alison; Jones, Daryl A.; Jones, Robert.

In: Critical Care and Resuscitation, Vol. 14, No. 3, 2012, p. 173-176.

Research output: Contribution to journalArticle

Bellomo, R, Suzuki, S, Marino, B, Starkey, GK, Chambers, B, Fink, MA, Wang, BZ, Houston, S, Eastwood, G, Calzavacca, P, Glassford, N, Skene, A, Jones, DA & Jones, R 2012, 'Normothermic extracorporeal perfusion of isolated porcine liver after warm ischaemia: A preliminary report', Critical Care and Resuscitation, vol. 14, no. 3, pp. 173-176.
Bellomo, Rinaldo ; Suzuki, Satoshi ; Marino, Bruno ; Starkey, Graeme K. ; Chambers, Brenton ; Fink, Michael A. ; Wang, Bao Zhong ; Houston, Shane ; Eastwood, Glenn ; Calzavacca, Paolo ; Glassford, Neil ; Skene, Alison ; Jones, Daryl A. ; Jones, Robert. / Normothermic extracorporeal perfusion of isolated porcine liver after warm ischaemia : A preliminary report. In: Critical Care and Resuscitation. 2012 ; Vol. 14, No. 3. pp. 173-176.
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