Abstract
The stimulatory effect of noradrenaline (NA) as well as oxytocin (OT) on bovine endometrial prostaglandin (PG) F(2α) production, and the intracellular mechanisms of their actions, were investigated in cultured bovine endometrial cells (a mixture of epithelial, stromal, and glandular cells). The cells were cultured in Dulbecco's Modified Eagle's medium and Ham's F-12 medium (1:1 [v:v]) with 10% calf serum. When the cells reached confluence, the culture medium was replaced with fresh medium with 0.1% BSA and various doses of NA (10-8-10-4 M). NA stimulated PGF(2α) production in a dose-dependent manner (p <0.05). To evaluate the intracellular mechanisms of NA and OT actions, the cells were treated with forskolin (an activator of adenylate cyclase), phorbol 12-myristate 13-acetate (PMA, an activator of protein kinase [PK] C), Rp-cAMP (a competitive cAMP antagonist and an inhibitor of PKA), U-73122 (an inhibitor of phospholipase [PL] C), or anthranilic acid (ACA, an inhibitor of PLA2). Forskolin and PMA stimulated PGF(2α) production in a dose-dependent manner (p <0.05). Rp-cAMP completely inhibited (p <0.001) the NA-induced, but not the OT-induced, PGF(2α) production. Although U-73122 inhibited only OT-induced PGF(2α) production (p <0.001), ACA completely stopped the actions of NA and OT. The overall results indicate that NA as well as OT is involved in the regulation of the endometrial PGF(2α) production in cattle and that the stimulatory effects of NA and OT on PGF(2α) production are mediated via the PKA and PKC pathways, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 277-282 |
| Number of pages | 6 |
| Journal | Biology of Reproduction |
| Volume | 60 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1999 |
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ASJC Scopus subject areas
- Cell Biology
- Developmental Biology
- Embryology
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Noradrenaline stimulates the production of prostaglandin F(2α) in cultured bovine endometrial cells. / Skarzynski, Dariusz J.; Uenoyama, Yoshihisa; Kotwica, Jan; Okuda, Kiyoshi.
In: Biology of Reproduction, Vol. 60, No. 2, 1999, p. 277-282.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Noradrenaline stimulates the production of prostaglandin F(2α) in cultured bovine endometrial cells
AU - Skarzynski, Dariusz J.
AU - Uenoyama, Yoshihisa
AU - Kotwica, Jan
AU - Okuda, Kiyoshi
PY - 1999
Y1 - 1999
N2 - The stimulatory effect of noradrenaline (NA) as well as oxytocin (OT) on bovine endometrial prostaglandin (PG) F(2α) production, and the intracellular mechanisms of their actions, were investigated in cultured bovine endometrial cells (a mixture of epithelial, stromal, and glandular cells). The cells were cultured in Dulbecco's Modified Eagle's medium and Ham's F-12 medium (1:1 [v:v]) with 10% calf serum. When the cells reached confluence, the culture medium was replaced with fresh medium with 0.1% BSA and various doses of NA (10-8-10-4 M). NA stimulated PGF(2α) production in a dose-dependent manner (p <0.05). To evaluate the intracellular mechanisms of NA and OT actions, the cells were treated with forskolin (an activator of adenylate cyclase), phorbol 12-myristate 13-acetate (PMA, an activator of protein kinase [PK] C), Rp-cAMP (a competitive cAMP antagonist and an inhibitor of PKA), U-73122 (an inhibitor of phospholipase [PL] C), or anthranilic acid (ACA, an inhibitor of PLA2). Forskolin and PMA stimulated PGF(2α) production in a dose-dependent manner (p <0.05). Rp-cAMP completely inhibited (p <0.001) the NA-induced, but not the OT-induced, PGF(2α) production. Although U-73122 inhibited only OT-induced PGF(2α) production (p <0.001), ACA completely stopped the actions of NA and OT. The overall results indicate that NA as well as OT is involved in the regulation of the endometrial PGF(2α) production in cattle and that the stimulatory effects of NA and OT on PGF(2α) production are mediated via the PKA and PKC pathways, respectively.
AB - The stimulatory effect of noradrenaline (NA) as well as oxytocin (OT) on bovine endometrial prostaglandin (PG) F(2α) production, and the intracellular mechanisms of their actions, were investigated in cultured bovine endometrial cells (a mixture of epithelial, stromal, and glandular cells). The cells were cultured in Dulbecco's Modified Eagle's medium and Ham's F-12 medium (1:1 [v:v]) with 10% calf serum. When the cells reached confluence, the culture medium was replaced with fresh medium with 0.1% BSA and various doses of NA (10-8-10-4 M). NA stimulated PGF(2α) production in a dose-dependent manner (p <0.05). To evaluate the intracellular mechanisms of NA and OT actions, the cells were treated with forskolin (an activator of adenylate cyclase), phorbol 12-myristate 13-acetate (PMA, an activator of protein kinase [PK] C), Rp-cAMP (a competitive cAMP antagonist and an inhibitor of PKA), U-73122 (an inhibitor of phospholipase [PL] C), or anthranilic acid (ACA, an inhibitor of PLA2). Forskolin and PMA stimulated PGF(2α) production in a dose-dependent manner (p <0.05). Rp-cAMP completely inhibited (p <0.001) the NA-induced, but not the OT-induced, PGF(2α) production. Although U-73122 inhibited only OT-induced PGF(2α) production (p <0.001), ACA completely stopped the actions of NA and OT. The overall results indicate that NA as well as OT is involved in the regulation of the endometrial PGF(2α) production in cattle and that the stimulatory effects of NA and OT on PGF(2α) production are mediated via the PKA and PKC pathways, respectively.
UR - http://www.scopus.com/inward/record.url?scp=0032894865&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032894865&partnerID=8YFLogxK
U2 - 10.1095/biolreprod60.2.277
DO - 10.1095/biolreprod60.2.277
M3 - Article
C2 - 9915991
AN - SCOPUS:0032894865
VL - 60
SP - 277
EP - 282
JO - Biology of Reproduction
JF - Biology of Reproduction
SN - 0006-3363
IS - 2
ER -