Abstract
Plants generally possess a strong ability to regenerate organs; for example, in tissue culture, shoots can regenerate from callus, a clump of actively proliferating, undifferentiated cells. Processing of pre-mRNA and ribosomal RNAs is important for callus formation and shoot regeneration. However, our knowledge of the roles of RNA quality control via the nonsense-mediated mRNA decay (NMD) pathway in shoot regeneration is limited. Here, we examined the shoot regeneration phenotypes of the low-beta-amylase1 (lba1)/upstream frame shift1-1 (upf1-1) and upf3-1 mutants, in which the core NMD components UPF1 and UPF3 are defective. These mutants formed callus from hypocotyl explants normally, but this callus behaved abnormally during shoot regeneration: the mutant callus generated numerous adventitious root structures instead of adventitious shoots in an auxin-dependent manner. Quantitative RT-PCR and microarray analyses showed that the upf mutations had widespread effects during culture on shoot-induction medium. In particular, the expression patterns of early auxin response genes, including those encoding AUXIN/INDOLE ACETIC ACID (AUX/IAA) family members, were significantly affected in the upf mutants. Also, the upregulation of shoot apical meristem-related transcription factor genes, such as CUP-SHAPED COTYLEDON1 (CUC1) and CUC2, was inhibited in the mutants. Taken together, these results indicate that NMD-mediated transcriptomic regulation modulates the auxin response in plants and thus plays crucial roles in the early stages of shoot regeneration.
| Original language | English |
|---|---|
| Pages (from-to) | 2000-2014 |
| Number of pages | 15 |
| Journal | Plant & cell physiology |
| Volume | 60 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Sep 1 2019 |
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Keywords
- Auxin response
- Nonsense-mediated mRNA decay
- Shoot apical meristem
- Shoot regeneration
- UPF
ASJC Scopus subject areas
- Physiology
- Plant Science
- Cell Biology
Cite this
Nonsense-Mediated mRNA Decay Deficiency Affects the Auxin Response and Shoot Regeneration in Arabidopsis. / Chiam, Nyet Cheng; Fujimura, Tomoyo; Sano, Ryosuke; Akiyoshi, Nobuhiro; Hiroyama, Ryoko; Watanabe, Yuichiro; Motose, Hiroyasu; Demura, Taku; Ohtani, Misato.
In: Plant & cell physiology, Vol. 60, No. 9, 01.09.2019, p. 2000-2014.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nonsense-Mediated mRNA Decay Deficiency Affects the Auxin Response and Shoot Regeneration in Arabidopsis
AU - Chiam, Nyet Cheng
AU - Fujimura, Tomoyo
AU - Sano, Ryosuke
AU - Akiyoshi, Nobuhiro
AU - Hiroyama, Ryoko
AU - Watanabe, Yuichiro
AU - Motose, Hiroyasu
AU - Demura, Taku
AU - Ohtani, Misato
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Plants generally possess a strong ability to regenerate organs; for example, in tissue culture, shoots can regenerate from callus, a clump of actively proliferating, undifferentiated cells. Processing of pre-mRNA and ribosomal RNAs is important for callus formation and shoot regeneration. However, our knowledge of the roles of RNA quality control via the nonsense-mediated mRNA decay (NMD) pathway in shoot regeneration is limited. Here, we examined the shoot regeneration phenotypes of the low-beta-amylase1 (lba1)/upstream frame shift1-1 (upf1-1) and upf3-1 mutants, in which the core NMD components UPF1 and UPF3 are defective. These mutants formed callus from hypocotyl explants normally, but this callus behaved abnormally during shoot regeneration: the mutant callus generated numerous adventitious root structures instead of adventitious shoots in an auxin-dependent manner. Quantitative RT-PCR and microarray analyses showed that the upf mutations had widespread effects during culture on shoot-induction medium. In particular, the expression patterns of early auxin response genes, including those encoding AUXIN/INDOLE ACETIC ACID (AUX/IAA) family members, were significantly affected in the upf mutants. Also, the upregulation of shoot apical meristem-related transcription factor genes, such as CUP-SHAPED COTYLEDON1 (CUC1) and CUC2, was inhibited in the mutants. Taken together, these results indicate that NMD-mediated transcriptomic regulation modulates the auxin response in plants and thus plays crucial roles in the early stages of shoot regeneration.
AB - Plants generally possess a strong ability to regenerate organs; for example, in tissue culture, shoots can regenerate from callus, a clump of actively proliferating, undifferentiated cells. Processing of pre-mRNA and ribosomal RNAs is important for callus formation and shoot regeneration. However, our knowledge of the roles of RNA quality control via the nonsense-mediated mRNA decay (NMD) pathway in shoot regeneration is limited. Here, we examined the shoot regeneration phenotypes of the low-beta-amylase1 (lba1)/upstream frame shift1-1 (upf1-1) and upf3-1 mutants, in which the core NMD components UPF1 and UPF3 are defective. These mutants formed callus from hypocotyl explants normally, but this callus behaved abnormally during shoot regeneration: the mutant callus generated numerous adventitious root structures instead of adventitious shoots in an auxin-dependent manner. Quantitative RT-PCR and microarray analyses showed that the upf mutations had widespread effects during culture on shoot-induction medium. In particular, the expression patterns of early auxin response genes, including those encoding AUXIN/INDOLE ACETIC ACID (AUX/IAA) family members, were significantly affected in the upf mutants. Also, the upregulation of shoot apical meristem-related transcription factor genes, such as CUP-SHAPED COTYLEDON1 (CUC1) and CUC2, was inhibited in the mutants. Taken together, these results indicate that NMD-mediated transcriptomic regulation modulates the auxin response in plants and thus plays crucial roles in the early stages of shoot regeneration.
KW - Auxin response
KW - Nonsense-mediated mRNA decay
KW - Shoot apical meristem
KW - Shoot regeneration
KW - UPF
UR - http://www.scopus.com/inward/record.url?scp=85072058466&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85072058466&partnerID=8YFLogxK
U2 - 10.1093/pcp/pcz154
DO - 10.1093/pcp/pcz154
M3 - Article
C2 - 31386149
AN - SCOPUS:85072058466
VL - 60
SP - 2000
EP - 2014
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 9
ER -