TY - JOUR
T1 - Nonsense-Mediated mRNA Decay Deficiency Affects the Auxin Response and Shoot Regeneration in Arabidopsis
AU - Chiam, Nyet Cheng
AU - Fujimura, Tomoyo
AU - Sano, Ryosuke
AU - Akiyoshi, Nobuhiro
AU - Hiroyama, Ryoko
AU - Watanabe, Yuichiro
AU - Motose, Hiroyasu
AU - Demura, Taku
AU - Ohtani, Misato
N1 - Funding Information:
We thank Dr. Harunori Kawabe, Dr. Yoichiro Watanabe, Ms. Mai Mukai, Ms. Ayumi Ihara, Ms. Shizuka Nishida, Ms. Eriko Tanaka, Ms. Yuki Mitsubayashi (Nara Institute of Science and Technology, Japan), Ms. Arika Takebayashi
Funding Information:
The RIKEN Centre for Sustainable Resource Science, The Naito Foundation, The Sumitomo Foundation [141267 to M.O.], Takeda Science Foundation, the Tomizawa Jun-ichi and Keiko Fund of Molecular Biology Society of Japan for Young Scientist, the Exploratory Research for Advanced Technology (ERATO) from Japan Science and Technology Agency (JST) [JPMJER1602 to M.O.], MEXT KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas ‘Plant-Structure Optimization Strategy’ [JP18H05484 and JP18H05489 to M.O. and T.D.] and JSPS KAKENHI [JP24770052, JP15H01235 and JP16K18569 to M.O.].
Publisher Copyright:
© 2019 The Author(s) 2019. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Plants generally possess a strong ability to regenerate organs; for example, in tissue culture, shoots can regenerate from callus, a clump of actively proliferating, undifferentiated cells. Processing of pre-mRNA and ribosomal RNAs is important for callus formation and shoot regeneration. However, our knowledge of the roles of RNA quality control via the nonsense-mediated mRNA decay (NMD) pathway in shoot regeneration is limited. Here, we examined the shoot regeneration phenotypes of the low-beta-amylase1 (lba1)/upstream frame shift1-1 (upf1-1) and upf3-1 mutants, in which the core NMD components UPF1 and UPF3 are defective. These mutants formed callus from hypocotyl explants normally, but this callus behaved abnormally during shoot regeneration: the mutant callus generated numerous adventitious root structures instead of adventitious shoots in an auxin-dependent manner. Quantitative RT-PCR and microarray analyses showed that the upf mutations had widespread effects during culture on shoot-induction medium. In particular, the expression patterns of early auxin response genes, including those encoding AUXIN/INDOLE ACETIC ACID (AUX/IAA) family members, were significantly affected in the upf mutants. Also, the upregulation of shoot apical meristem-related transcription factor genes, such as CUP-SHAPED COTYLEDON1 (CUC1) and CUC2, was inhibited in the mutants. Taken together, these results indicate that NMD-mediated transcriptomic regulation modulates the auxin response in plants and thus plays crucial roles in the early stages of shoot regeneration.
AB - Plants generally possess a strong ability to regenerate organs; for example, in tissue culture, shoots can regenerate from callus, a clump of actively proliferating, undifferentiated cells. Processing of pre-mRNA and ribosomal RNAs is important for callus formation and shoot regeneration. However, our knowledge of the roles of RNA quality control via the nonsense-mediated mRNA decay (NMD) pathway in shoot regeneration is limited. Here, we examined the shoot regeneration phenotypes of the low-beta-amylase1 (lba1)/upstream frame shift1-1 (upf1-1) and upf3-1 mutants, in which the core NMD components UPF1 and UPF3 are defective. These mutants formed callus from hypocotyl explants normally, but this callus behaved abnormally during shoot regeneration: the mutant callus generated numerous adventitious root structures instead of adventitious shoots in an auxin-dependent manner. Quantitative RT-PCR and microarray analyses showed that the upf mutations had widespread effects during culture on shoot-induction medium. In particular, the expression patterns of early auxin response genes, including those encoding AUXIN/INDOLE ACETIC ACID (AUX/IAA) family members, were significantly affected in the upf mutants. Also, the upregulation of shoot apical meristem-related transcription factor genes, such as CUP-SHAPED COTYLEDON1 (CUC1) and CUC2, was inhibited in the mutants. Taken together, these results indicate that NMD-mediated transcriptomic regulation modulates the auxin response in plants and thus plays crucial roles in the early stages of shoot regeneration.
KW - Auxin response
KW - Nonsense-mediated mRNA decay
KW - Shoot apical meristem
KW - Shoot regeneration
KW - UPF
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U2 - 10.1093/pcp/pcz154
DO - 10.1093/pcp/pcz154
M3 - Article
C2 - 31386149
AN - SCOPUS:85072058466
VL - 60
SP - 2000
EP - 2014
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 9
ER -