Nonsense-Mediated mRNA Decay Deficiency Affects the Auxin Response and Shoot Regeneration in Arabidopsis

Nyet Cheng Chiam, Tomoyo Fujimura, Ryosuke Sano, Nobuhiro Akiyoshi, Ryoko Hiroyama, Yuichiro Watanabe, Hiroyasu Motose, Taku Demura, Misato Ohtani

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Plants generally possess a strong ability to regenerate organs; for example, in tissue culture, shoots can regenerate from callus, a clump of actively proliferating, undifferentiated cells. Processing of pre-mRNA and ribosomal RNAs is important for callus formation and shoot regeneration. However, our knowledge of the roles of RNA quality control via the nonsense-mediated mRNA decay (NMD) pathway in shoot regeneration is limited. Here, we examined the shoot regeneration phenotypes of the low-beta-amylase1 (lba1)/upstream frame shift1-1 (upf1-1) and upf3-1 mutants, in which the core NMD components UPF1 and UPF3 are defective. These mutants formed callus from hypocotyl explants normally, but this callus behaved abnormally during shoot regeneration: the mutant callus generated numerous adventitious root structures instead of adventitious shoots in an auxin-dependent manner. Quantitative RT-PCR and microarray analyses showed that the upf mutations had widespread effects during culture on shoot-induction medium. In particular, the expression patterns of early auxin response genes, including those encoding AUXIN/INDOLE ACETIC ACID (AUX/IAA) family members, were significantly affected in the upf mutants. Also, the upregulation of shoot apical meristem-related transcription factor genes, such as CUP-SHAPED COTYLEDON1 (CUC1) and CUC2, was inhibited in the mutants. Taken together, these results indicate that NMD-mediated transcriptomic regulation modulates the auxin response in plants and thus plays crucial roles in the early stages of shoot regeneration.

Original languageEnglish
Pages (from-to)2000-2014
Number of pages15
JournalPlant & cell physiology
Volume60
Issue number9
DOIs
Publication statusPublished - Sep 1 2019

Keywords

  • Auxin response
  • Nonsense-mediated mRNA decay
  • Shoot apical meristem
  • Shoot regeneration
  • UPF

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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    Chiam, N. C., Fujimura, T., Sano, R., Akiyoshi, N., Hiroyama, R., Watanabe, Y., Motose, H., Demura, T., & Ohtani, M. (2019). Nonsense-Mediated mRNA Decay Deficiency Affects the Auxin Response and Shoot Regeneration in Arabidopsis. Plant & cell physiology, 60(9), 2000-2014. https://doi.org/10.1093/pcp/pcz154