Nonredundant functions of Arabidopsis LecRK-V.2 and LecRK-VII.1 in controlling stomatal immunity and jasmonate-mediated stomatal closure

Shweta Yekondi, Fu Chun Liang, Eiji Okuma, Amandine Radziejwoski, Hsien Wei Mai, Swadhin Swain, Prashant Singh, Mathieu Gauthier, Hsiao Chiao Chien, Yoshiyuki Murata, Laurent Zimmerli

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4 Citations (Scopus)

Abstract

Stomatal immunity restricts bacterial entry to leaves through the recognition of microbe-associated molecular patterns (MAMPs) by pattern-recognition receptors (PRRs) and downstream abscisic acid and salicylic acid signaling. Through a reverse genetics approach, we characterized the function of the L-type lectin receptor kinase-V.2 (LecRK-V.2) and -VII.1 (LecRK-VII.1). Analyses of interactions with the PRR FLAGELLIN SENSING2 (FLS2) were performed by co-immunoprecipitation and bimolecular fluorescence complementation and whole-cell patch-clamp analyses were used to evaluate guard cell Ca2+-permeable cation channels. The Arabidopsis thaliana LecRK-V.2 and LecRK-VII.1 and notably their kinase activities were required for full activation of stomatal immunity. Knockout lecrk-V.2 and lecrk-VII.1 mutants were hyper-susceptible to Pseudomonas syringae infection and showed defective stomatal closure in response to bacteria or to the MAMPs flagellin and EF-Tu. By contrast, Arabidopsis over-expressing LecRK-V.2 or LecRK-VII.1 demonstrated a potentiated stomatal immunity. LecRK-V.2 and LecRK-VII.1 are shown to be part of the FLS2 PRR complex. In addition, LecRK-V.2 and LecRK-VII.1 were critical for methyl jasmonate (MeJA)-mediated stomatal closure, notably for MeJA-induced activation of guard cell Ca2+-permeable cation channels. This study highlights the role of LecRK-V.2 and LecRK-VII.1 in stomatal immunity at the FLS2 PRR complex and in MeJA-mediated stomatal closure.

Original languageEnglish
JournalNew Phytologist
DOIs
Publication statusAccepted/In press - Jan 1 2017

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Keywords

  • Abscisic acid
  • Arabidopsis thaliana
  • Defense
  • Hormone
  • Jasmonate
  • Lectin receptor kinase
  • Salicylic acid
  • Stomatal immunity

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Yekondi, S., Liang, F. C., Okuma, E., Radziejwoski, A., Mai, H. W., Swain, S., Singh, P., Gauthier, M., Chien, H. C., Murata, Y., & Zimmerli, L. (Accepted/In press). Nonredundant functions of Arabidopsis LecRK-V.2 and LecRK-VII.1 in controlling stomatal immunity and jasmonate-mediated stomatal closure. New Phytologist. https://doi.org/10.1111/nph.14953