Nonredundant functions of Arabidopsis LecRK-V.2 and LecRK-VII.1 in controlling stomatal immunity and jasmonate-mediated stomatal closure

Shweta Yekondi, Fu Chun Liang, Eiji Okuma, Amandine Radziejwoski, Hsien Wei Mai, Swadhin Swain, Prashant Singh, Mathieu Gauthier, Hsiao Chiao Chien, Yoshiyuki Murata, Laurent Zimmerli

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Stomatal immunity restricts bacterial entry to leaves through the recognition of microbe-associated molecular patterns (MAMPs) by pattern-recognition receptors (PRRs) and downstream abscisic acid and salicylic acid signaling. Through a reverse genetics approach, we characterized the function of the L-type lectin receptor kinase-V.2 (LecRK-V.2) and -VII.1 (LecRK-VII.1). Analyses of interactions with the PRR FLAGELLIN SENSING2 (FLS2) were performed by co-immunoprecipitation and bimolecular fluorescence complementation and whole-cell patch-clamp analyses were used to evaluate guard cell Ca2+-permeable cation channels. The Arabidopsis thaliana LecRK-V.2 and LecRK-VII.1 and notably their kinase activities were required for full activation of stomatal immunity. Knockout lecrk-V.2 and lecrk-VII.1 mutants were hyper-susceptible to Pseudomonas syringae infection and showed defective stomatal closure in response to bacteria or to the MAMPs flagellin and EF-Tu. By contrast, Arabidopsis over-expressing LecRK-V.2 or LecRK-VII.1 demonstrated a potentiated stomatal immunity. LecRK-V.2 and LecRK-VII.1 are shown to be part of the FLS2 PRR complex. In addition, LecRK-V.2 and LecRK-VII.1 were critical for methyl jasmonate (MeJA)-mediated stomatal closure, notably for MeJA-induced activation of guard cell Ca2+-permeable cation channels. This study highlights the role of LecRK-V.2 and LecRK-VII.1 in stomatal immunity at the FLS2 PRR complex and in MeJA-mediated stomatal closure.

Original languageEnglish
JournalNew Phytologist
DOIs
Publication statusAccepted/In press - Jan 1 2017

Fingerprint

Pattern Recognition Receptors
Arabidopsis
Immunity
immunity
methyl jasmonate
receptors
guard cells
Cations
Phosphotransferases
Mitogen Receptors
Pseudomonas syringae
cations
Flagellin
phosphotransferases (kinases)
Reverse Genetics
Pseudomonas Infections
Abscisic Acid
Salicylic Acid
microorganisms
calcium

Keywords

  • Abscisic acid
  • Arabidopsis thaliana
  • Defense
  • Hormone
  • Jasmonate
  • Lectin receptor kinase
  • Salicylic acid
  • Stomatal immunity

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Nonredundant functions of Arabidopsis LecRK-V.2 and LecRK-VII.1 in controlling stomatal immunity and jasmonate-mediated stomatal closure. / Yekondi, Shweta; Liang, Fu Chun; Okuma, Eiji; Radziejwoski, Amandine; Mai, Hsien Wei; Swain, Swadhin; Singh, Prashant; Gauthier, Mathieu; Chien, Hsiao Chiao; Murata, Yoshiyuki; Zimmerli, Laurent.

In: New Phytologist, 01.01.2017.

Research output: Contribution to journalArticle

Yekondi, Shweta ; Liang, Fu Chun ; Okuma, Eiji ; Radziejwoski, Amandine ; Mai, Hsien Wei ; Swain, Swadhin ; Singh, Prashant ; Gauthier, Mathieu ; Chien, Hsiao Chiao ; Murata, Yoshiyuki ; Zimmerli, Laurent. / Nonredundant functions of Arabidopsis LecRK-V.2 and LecRK-VII.1 in controlling stomatal immunity and jasmonate-mediated stomatal closure. In: New Phytologist. 2017.
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AU - Liang, Fu Chun

AU - Okuma, Eiji

AU - Radziejwoski, Amandine

AU - Mai, Hsien Wei

AU - Swain, Swadhin

AU - Singh, Prashant

AU - Gauthier, Mathieu

AU - Chien, Hsiao Chiao

AU - Murata, Yoshiyuki

AU - Zimmerli, Laurent

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AB - Stomatal immunity restricts bacterial entry to leaves through the recognition of microbe-associated molecular patterns (MAMPs) by pattern-recognition receptors (PRRs) and downstream abscisic acid and salicylic acid signaling. Through a reverse genetics approach, we characterized the function of the L-type lectin receptor kinase-V.2 (LecRK-V.2) and -VII.1 (LecRK-VII.1). Analyses of interactions with the PRR FLAGELLIN SENSING2 (FLS2) were performed by co-immunoprecipitation and bimolecular fluorescence complementation and whole-cell patch-clamp analyses were used to evaluate guard cell Ca2+-permeable cation channels. The Arabidopsis thaliana LecRK-V.2 and LecRK-VII.1 and notably their kinase activities were required for full activation of stomatal immunity. Knockout lecrk-V.2 and lecrk-VII.1 mutants were hyper-susceptible to Pseudomonas syringae infection and showed defective stomatal closure in response to bacteria or to the MAMPs flagellin and EF-Tu. By contrast, Arabidopsis over-expressing LecRK-V.2 or LecRK-VII.1 demonstrated a potentiated stomatal immunity. LecRK-V.2 and LecRK-VII.1 are shown to be part of the FLS2 PRR complex. In addition, LecRK-V.2 and LecRK-VII.1 were critical for methyl jasmonate (MeJA)-mediated stomatal closure, notably for MeJA-induced activation of guard cell Ca2+-permeable cation channels. This study highlights the role of LecRK-V.2 and LecRK-VII.1 in stomatal immunity at the FLS2 PRR complex and in MeJA-mediated stomatal closure.

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