Noninvasive evaluation of nicotinic acetylcholine receptor availability in mouse brain using single-photon emission computed tomography with [123I]5IA

Yuki Matsuura, Masashi Ueda, Yusuke Higaki, Keiko Watanabe, Shogo Habara, Shinichiro Kamino, Hideo Saji, Shuichi Enomoto

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Introduction: Nicotinic acetylcholine receptors (nAChRs) are of great interest because they are implicated in higher brain functions. Nuclear medical imaging is one of the useful techniques for noninvasive evaluation of physiological and pathological function in living subjects. Recent progress in nuclear medical imaging modalities enables the clear visualization of the organs of small rodents. Thus, translational research using nuclear medical imaging in transgenic mice has become possible and helps to elucidate human disease pathology. However, imaging of α4β2 nAChRs in the mouse brain has not yet been performed. The purpose of this study was to assess the feasibility of single-photon emission computed tomography (SPECT) with 5-[123I]iodo-3-[2(S)-azetidinylmethoxy]pyridine ([123I]5IA) for evaluating α4β2 nAChR availability in the mouse brain. Methods: A 60-min dynamic SPECT imaging session of α4β2 nAChRs in the mouse brain was performed. The regional distribution of radioactivity in the SPECT images was compared to the density of α4β2 nAChRs measured in an identical mouse. Alteration of nAChR density in the brains of Tg2576 mice was also evaluated. Results: The mouse brain was clearly visualized by [123I]5IA-SPECT and probe accumulation was significantly inhibited by pretreatment with (-)-nicotine. The regional distribution of radioactivity in SPECT images showed a significant positive correlation with α4β2 nAChR density measured in an identical mouse brain. Moreover, [123I]5IA-SPECT was able to detect the up-regulation of α4β2 nAChRs in the brains of Tg2576 transgenic mice. Conclusions: [123I]5IA-SPECT imaging would be a promising tool for evaluating α4β2 nAChR availability in the mouse brain and may be useful in translational research focused on nAChR-related diseases.

Original languageEnglish
Pages (from-to)372-378
Number of pages7
JournalNuclear Medicine and Biology
Volume43
Issue number6
DOIs
Publication statusPublished - Jun 1 2016

Fingerprint

Nicotinic Receptors
Single-Photon Emission-Computed Tomography
Brain
Diagnostic Imaging
Translational Medical Research
Radioactivity
Transgenic Mice
Nicotine
Rodentia
Up-Regulation
Pathology

Keywords

  • 5-[I]iodo-3-(2(S)-azetidinylmethoxy)pyridine ([I]5IA)
  • Alzheimer's disease
  • Mouse
  • Nicotinic acetylcholine receptor (nAChR)
  • Single-photon emission computed tomography (SPECT)
  • Tg2576

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging

Cite this

Noninvasive evaluation of nicotinic acetylcholine receptor availability in mouse brain using single-photon emission computed tomography with [123I]5IA. / Matsuura, Yuki; Ueda, Masashi; Higaki, Yusuke; Watanabe, Keiko; Habara, Shogo; Kamino, Shinichiro; Saji, Hideo; Enomoto, Shuichi.

In: Nuclear Medicine and Biology, Vol. 43, No. 6, 01.06.2016, p. 372-378.

Research output: Contribution to journalArticle

Matsuura, Yuki ; Ueda, Masashi ; Higaki, Yusuke ; Watanabe, Keiko ; Habara, Shogo ; Kamino, Shinichiro ; Saji, Hideo ; Enomoto, Shuichi. / Noninvasive evaluation of nicotinic acetylcholine receptor availability in mouse brain using single-photon emission computed tomography with [123I]5IA. In: Nuclear Medicine and Biology. 2016 ; Vol. 43, No. 6. pp. 372-378.
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AU - Habara, Shogo

AU - Kamino, Shinichiro

AU - Saji, Hideo

AU - Enomoto, Shuichi

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