Non-ulcerative cutaneous lesion in immunodeficient mice with Leishmania amazonensis infection

Takashi Kuramochi, Toshimitsu Hatabu, Mamoru Ito, Yoshihito Ueyama, Shin Ichiro Kawazu, Takashi Onodera, Yoshitsugu Matsumoto

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Cutaneous leishmaniasis begins as papules or nodules at the site of promastigote inoculation. The next key pathogenic event in this disease is the formation of an ulcer at this site. Leishmania infection in immunodeficient mice, however, showed non-ulcerative cutaneous lesions suggesting the involvement of the immune system in ulcer formation. Severe combined immunodeficient (SCID), recombination-activating gene 2 knockout (RAG-2(-/-)), and immunocompetent mice were inoculated subcutaneously with cultured L. amazonensis promastigotes. Macroscopic nodules appeared at the inoculation site within 2 weeks of infection in all the mice and gradually extended to the surrounding skin tissue. Although nodules of immunocompetent mice ulcerated within 6 weeks, immunodeficient mice did not form ulcers even after 25 weeks of inoculation. These results strongly suggest the importance of functional T and B cells in ulcer formation of cutaneous leishmaniasis and are consistent with clinical features of non-ulcerative cutaneous leishmaniasis in some AIDS patients. The present study also indicates that the L. amazonensis-infected immunodeficient mouse model might be suitable for studying the mechanisms of ulcer formation in cutaneous leishmaniasis.

Original languageEnglish
Pages (from-to)47-53
Number of pages7
JournalParasitology International
Volume48
Issue number1
DOIs
Publication statusPublished - Mar 1 1999
Externally publishedYes

Keywords

  • Immunodeficient mice
  • Leishmania
  • Non-ulcerative lesion

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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