Non-thiol-activated property of a cholesterol-binding hemolysin produced by Vibrio vulnificus

Shin-ichi Miyoshi, Hiroyasu Yamanaka, Noriko Miyoshi, Sumio Shinoda

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Vibrio vulnificus hemolysin (VVH) and streptolysin O (SLO) are both cholesterol-binding hemolysins. Both hemolysins were inactivated with H2O2, but the lost activity of SLO was restored by addition of thiol compounds, whereas that of VVH was not. Moreover, the activity of VVH was lowered by thiol compounds but not by thiolblocking agents, whereas the latter produced a decrease in SLO activity. These results suggest that VVH is not a thiol-activated hemolysin, in spite of its cholesterol-binding property.

Original languageEnglish
Pages (from-to)213-216
Number of pages4
JournalFEMS Microbiology Letters
Volume30
Issue number1-2
Publication statusPublished - 1985

Fingerprint

Vibrio vulnificus
Hemolysin Proteins
Cholesterol
Sulfhydryl Compounds
Vibrio hemolysin
streptolysin O

Keywords

  • cytolysin
  • Marine vibrio
  • oxygen-labile hemolysin

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Non-thiol-activated property of a cholesterol-binding hemolysin produced by Vibrio vulnificus. / Miyoshi, Shin-ichi; Yamanaka, Hiroyasu; Miyoshi, Noriko; Shinoda, Sumio.

In: FEMS Microbiology Letters, Vol. 30, No. 1-2, 1985, p. 213-216.

Research output: Contribution to journalArticle

Miyoshi, Shin-ichi ; Yamanaka, Hiroyasu ; Miyoshi, Noriko ; Shinoda, Sumio. / Non-thiol-activated property of a cholesterol-binding hemolysin produced by Vibrio vulnificus. In: FEMS Microbiology Letters. 1985 ; Vol. 30, No. 1-2. pp. 213-216.
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