Abstract
Fermentation of lignocellulosic biomass requires auxiliary materials, including nutrients, to ensure the proliferation of microorganisms. Nutrients are usually inexpensive, but their contribution to the cost is considerable because of the very low prices of fermentation products, such as bio-ethanol. Using substances present in native lignocellulosic biomass as nutrients for fermentation was proposed and demonstrated. Leaves and stalks of corn plants were used as biomass, and nutrients were recovered as a nutrient solution by soaking them in water before alkaline peroxide pretreatment. Pretreated biomass and the nutrient solution derived from the same lot were used for non-sterile simultaneous enzymatic saccharification and thermophilic l-lactic acid fermentation (SSF). Using the nutrient solution in the saccharification step did not impact sugar recovery, and instead improved sugar yields because of the presence of eluted sugars in the solution. The l-lactic acid yield of 0.33 g g−1 based on native biomass weight indicated that the nutrient solution functioned as a source of nutrients and sugars, especially as a source of essential phosphorus. Comparatively, autoclaved SSF yielded less or no l-lactic acid, indicating an apparent inhibitive effect derived from the nutrient solution on bacterial growth.
| Original language | English |
|---|---|
| Journal | Journal of Material Cycles and Waste Management |
| DOIs | |
| Publication status | Accepted/In press - Apr 22 2015 |
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Keywords
- Bacillus coagulans
- Catch crop
- Corn stover
- Enzymatic saccharification
- Nutrients
ASJC Scopus subject areas
- Waste Management and Disposal
- Mechanics of Materials
Cite this
Non-sterile simultaneous saccharification and fermentation of corn leaves and stalks to l-lactic acid without external nutrient addition. / Akao, Satoshi; Nagare, Hideaki; Maeda, Morihiro; Kondo, Keisuke; Fujiwara, Taku.
In: Journal of Material Cycles and Waste Management, 22.04.2015.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Non-sterile simultaneous saccharification and fermentation of corn leaves and stalks to l-lactic acid without external nutrient addition
AU - Akao, Satoshi
AU - Nagare, Hideaki
AU - Maeda, Morihiro
AU - Kondo, Keisuke
AU - Fujiwara, Taku
PY - 2015/4/22
Y1 - 2015/4/22
N2 - Fermentation of lignocellulosic biomass requires auxiliary materials, including nutrients, to ensure the proliferation of microorganisms. Nutrients are usually inexpensive, but their contribution to the cost is considerable because of the very low prices of fermentation products, such as bio-ethanol. Using substances present in native lignocellulosic biomass as nutrients for fermentation was proposed and demonstrated. Leaves and stalks of corn plants were used as biomass, and nutrients were recovered as a nutrient solution by soaking them in water before alkaline peroxide pretreatment. Pretreated biomass and the nutrient solution derived from the same lot were used for non-sterile simultaneous enzymatic saccharification and thermophilic l-lactic acid fermentation (SSF). Using the nutrient solution in the saccharification step did not impact sugar recovery, and instead improved sugar yields because of the presence of eluted sugars in the solution. The l-lactic acid yield of 0.33 g g−1 based on native biomass weight indicated that the nutrient solution functioned as a source of nutrients and sugars, especially as a source of essential phosphorus. Comparatively, autoclaved SSF yielded less or no l-lactic acid, indicating an apparent inhibitive effect derived from the nutrient solution on bacterial growth.
AB - Fermentation of lignocellulosic biomass requires auxiliary materials, including nutrients, to ensure the proliferation of microorganisms. Nutrients are usually inexpensive, but their contribution to the cost is considerable because of the very low prices of fermentation products, such as bio-ethanol. Using substances present in native lignocellulosic biomass as nutrients for fermentation was proposed and demonstrated. Leaves and stalks of corn plants were used as biomass, and nutrients were recovered as a nutrient solution by soaking them in water before alkaline peroxide pretreatment. Pretreated biomass and the nutrient solution derived from the same lot were used for non-sterile simultaneous enzymatic saccharification and thermophilic l-lactic acid fermentation (SSF). Using the nutrient solution in the saccharification step did not impact sugar recovery, and instead improved sugar yields because of the presence of eluted sugars in the solution. The l-lactic acid yield of 0.33 g g−1 based on native biomass weight indicated that the nutrient solution functioned as a source of nutrients and sugars, especially as a source of essential phosphorus. Comparatively, autoclaved SSF yielded less or no l-lactic acid, indicating an apparent inhibitive effect derived from the nutrient solution on bacterial growth.
KW - Bacillus coagulans
KW - Catch crop
KW - Corn stover
KW - Enzymatic saccharification
KW - Nutrients
UR - http://www.scopus.com/inward/record.url?scp=84928139054&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84928139054&partnerID=8YFLogxK
U2 - 10.1007/s10163-015-0390-y
DO - 10.1007/s10163-015-0390-y
M3 - Article
AN - SCOPUS:84928139054
JO - Journal of Material Cycles and Waste Management
JF - Journal of Material Cycles and Waste Management
SN - 1438-4957
ER -