Non-coding RNA Neat1 and Abhd11os expressions are dysregulated in medium spiny neurons of Huntington disease model mice

Hongsun Park; Haruko Miyazaki; Tomoyuki Yamanaka; Nobuyuki Nukina

doi:10.1016/j.neures.2018.10.013

Non-coding RNA Neat1 and Abhd11os expressions are dysregulated in medium spiny neurons of Huntington disease model mice

Hongsun Park, Haruko Miyazaki, Tomoyuki Yamanaka, Nobuyuki Nukina

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Huntington Disease (HD) is a neurodegenerative disorder caused by expanded CAG repeats in the exon1 of huntingtin gene (HTT). The mutant HTT affects the transcriptional profile of neurons by disrupting the activities of transcriptional machinery and alters expression of many genes. In this study, we identified dysregulated non-coding RNAs (ncRNAs) in medium spiny neurons of 4-week-old HD model mouse. Also, we observed the intracellular localizations of Abhd11os and Neat1 ncRNAs by ViewRNA in situ hybridization, which could provide more precise detection, suggesting that it is a useful method to investigate the expression changes of genes with low expression levels.

Original language	English
Pages (from-to)	58-63
Number of pages	6
Journal	Neuroscience Research
Volume	147
DOIs	https://doi.org/10.1016/j.neures.2018.10.013
Publication status	Published - Oct 2019
Externally published	Yes

Keywords

Abhd11os
Gene expression
Huntington disease
Microarray
Neat1
Non-coding RNA
ViewRNA in situ hybridization

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/j.neures.2018.10.013

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title = "Non-coding RNA Neat1 and Abhd11os expressions are dysregulated in medium spiny neurons of Huntington disease model mice",

abstract = "Huntington Disease (HD) is a neurodegenerative disorder caused by expanded CAG repeats in the exon1 of huntingtin gene (HTT). The mutant HTT affects the transcriptional profile of neurons by disrupting the activities of transcriptional machinery and alters expression of many genes. In this study, we identified dysregulated non-coding RNAs (ncRNAs) in medium spiny neurons of 4-week-old HD model mouse. Also, we observed the intracellular localizations of Abhd11os and Neat1 ncRNAs by ViewRNA in situ hybridization, which could provide more precise detection, suggesting that it is a useful method to investigate the expression changes of genes with low expression levels.",

keywords = "Abhd11os, Gene expression, Huntington disease, Microarray, Neat1, Non-coding RNA, ViewRNA in situ hybridization",

author = "Hongsun Park and Haruko Miyazaki and Tomoyuki Yamanaka and Nobuyuki Nukina",

note = "Funding Information: This work was supported by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan to N.N. (grant numbers 25253066 , 17H01564 , 16H01345 ), to T.Y. ( 18H02723 , 17KT0131 ), and to H.M. ( 16K07005 ). Funding Information: This work was supported by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan to N.N. (grant numbers 25253066, 17H01564, 16H01345), to T.Y. (18H02723, 17KT0131), and to H.M. (16K07005). The authors thank UY Edward William KO for English editing of the manuscript. Publisher Copyright: {\textcopyright} 2018 Elsevier B.V. and Japan Neuroscience Society",

year = "2019",

month = oct,

doi = "10.1016/j.neures.2018.10.013",

language = "English",

volume = "147",

pages = "58--63",

journal = "Neuroscience Research",

issn = "0168-0102",

publisher = "Elsevier Ireland Ltd",

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TY - JOUR

T1 - Non-coding RNA Neat1 and Abhd11os expressions are dysregulated in medium spiny neurons of Huntington disease model mice

AU - Park, Hongsun

AU - Miyazaki, Haruko

AU - Yamanaka, Tomoyuki

AU - Nukina, Nobuyuki

N1 - Funding Information: This work was supported by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan to N.N. (grant numbers 25253066 , 17H01564 , 16H01345 ), to T.Y. ( 18H02723 , 17KT0131 ), and to H.M. ( 16K07005 ). Funding Information: This work was supported by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan to N.N. (grant numbers 25253066, 17H01564, 16H01345), to T.Y. (18H02723, 17KT0131), and to H.M. (16K07005). The authors thank UY Edward William KO for English editing of the manuscript. Publisher Copyright: © 2018 Elsevier B.V. and Japan Neuroscience Society

PY - 2019/10

Y1 - 2019/10

N2 - Huntington Disease (HD) is a neurodegenerative disorder caused by expanded CAG repeats in the exon1 of huntingtin gene (HTT). The mutant HTT affects the transcriptional profile of neurons by disrupting the activities of transcriptional machinery and alters expression of many genes. In this study, we identified dysregulated non-coding RNAs (ncRNAs) in medium spiny neurons of 4-week-old HD model mouse. Also, we observed the intracellular localizations of Abhd11os and Neat1 ncRNAs by ViewRNA in situ hybridization, which could provide more precise detection, suggesting that it is a useful method to investigate the expression changes of genes with low expression levels.

AB - Huntington Disease (HD) is a neurodegenerative disorder caused by expanded CAG repeats in the exon1 of huntingtin gene (HTT). The mutant HTT affects the transcriptional profile of neurons by disrupting the activities of transcriptional machinery and alters expression of many genes. In this study, we identified dysregulated non-coding RNAs (ncRNAs) in medium spiny neurons of 4-week-old HD model mouse. Also, we observed the intracellular localizations of Abhd11os and Neat1 ncRNAs by ViewRNA in situ hybridization, which could provide more precise detection, suggesting that it is a useful method to investigate the expression changes of genes with low expression levels.

KW - Abhd11os

KW - Gene expression

KW - Huntington disease

KW - Microarray

KW - Neat1

KW - Non-coding RNA

KW - ViewRNA in situ hybridization

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Non-coding RNA Neat1 and Abhd11os expressions are dysregulated in medium spiny neurons of Huntington disease model mice

Abstract

Keywords

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