NMR signal assignment of the polyuridine tract of the single-stranded RNA complexed with Sxl RBD1-RBD2 by using residue selective [5-(2)H]uridine substitutions.

I. Kim, Y. Muto, K. Hosono, A. Kitamura, S. Watanabe, T. Ohtsuki, G. Kawai, H. Takaku, K. Watanabe, H. Sakamoto, Y. Shimura, S. Yokoyama

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Abstract

Using [5-(2)H]uridine phosphoramidite, we synthesized a series of 2H-labeled Drosophila Sex-lethal (Sxl) target RNAs, in which all the uridine residues except one were specifically replaced by [5-(2)H]uridine. By observing the H5-H6 cross peaks of RNA in the TOCSY spectra, we unambiguously assigned all the base proton resonances of the target RNA in a Sxl x RNA complex. Furthermore, it was shown that Sxl differently recognizes A and G in a position prior to the polyuridine tract.

Original languageEnglish
Pages (from-to)203-204
Number of pages2
JournalNucleic acids symposium series
Issue number42
DOIs
Publication statusPublished - 1999

ASJC Scopus subject areas

  • Medicine(all)

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    Kim, I., Muto, Y., Hosono, K., Kitamura, A., Watanabe, S., Ohtsuki, T., Kawai, G., Takaku, H., Watanabe, K., Sakamoto, H., Shimura, Y., & Yokoyama, S. (1999). NMR signal assignment of the polyuridine tract of the single-stranded RNA complexed with Sxl RBD1-RBD2 by using residue selective [5-(2)H]uridine substitutions. Nucleic acids symposium series, (42), 203-204. https://doi.org/10.1093/nass/42.1.203