NLRP3 inflammasome expression in lipopolysaccharide-induced otitis media

Shin Kariya, Mitsuhiro Okano, Pengfei Zhao, Yukihide Maeda, Yuko Kataoka, Takaya Higaki, Yohei Noda, Seiichiro Makihara, Kazunori Nishizaki

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2 Citations (Scopus)

Abstract

Background: The nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome is a critical molecule mediating interleukin-1β (IL-1β) responses. However, the role of the NLRP3 inflammasome in otitis media has not been fully examined. Aims/Objectives: The purpose of this study is to assess the expression of NLRP3, ASC (apoptosis-associated speck-like protein containing a caspase recruitment domain and a pyrin domain), and caspase-1 in lipopolysaccharide-induced otitis media. Materials and methods: BALB/c mice received a transtympanic injection of either lipopolysaccharide or phosphate-buffered saline. The mice were sacrificed 24 h after injection. Concentrations of IL-1β, NLRP3, ASC, and caspase-1 in the middle ear effusions were measured by enzyme-linked immunosorbent assay. Temporal bones were processed for histologic examination and immunohistochemistry. Results: The transtympanic injection of lipopolysaccharide significantly upregulated levels of IL-1β, NLRP3, ASC, and caspase-1 in the middle ear as compared with the control mice. The proteins of NLRP3, ASC, and caspase-1 were observed in infiltrating inflammatory cells induced by lipopolysaccharide in the middle ear cavity. Conclusions and significance: Lipopolysaccharide induces NLRP3 inflammasome components in the middle ear. The NLRP3 inflammasome may play an important role in the pathogenesis of otitis media. Modulation of inflammasome-mediated inflammation may be a novel therapeutic strategy for otitis media.

Original languageEnglish
JournalActa Oto-Laryngologica
DOIs
Publication statusAccepted/In press - Jan 1 2019

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Keywords

  • cytokine
  • Infection
  • inflammation
  • interleukin
  • NOD-like receptor
  • toll-like receptor

ASJC Scopus subject areas

  • Otorhinolaryngology

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