Nitrogen recycling and remobilization are differentially controlled by leaf senescence and development stage in Arabidopsis under low nitrogen nutrition

Céline Diaz, Thomas Lemaître, Aurélie Christ, Marianne Azzopardi, Yusuke Kato, Fumihiko Sato, Jean Francxois Morot-Gaudry, Frédérik Le Dily, Céline Masclaux-Daubresse

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158 Citations (Scopus)

Abstract

Five recombinant inbred lines (RILs) of Arabidopsis (Arabidopsis thaliana), previously selected from the Bay-0 x Shahdara RIL population on the basis of differential leaf senescence phenotypes (from early senescing to late senescing) when cultivated under nitrogen (N)-limiting conditions, were analyzed to monitor metabolic markers related to N assimilation and N remobilization pathways. In each RIL, a decrease of total N, free amino acid, and soluble protein contents with leaf aging was observed. In parallel, the expression of markers for N remobilization such as cytosolic glutamine synthetase, glutamate dehydrogenase, and CND41-like protease was increased. This increase occurred earlier and more rapidly in early-senescing lines than in late-senescing lines. We measured the partitioning of 15N between sink and source leaves during the vegetative stage of development using 15N tracing and showed that N remobilization from the source leaves to the sink leaves was more efficient in the early-senescing lines. The N remobilization rate was correlated with leaf senescence severity at the vegetative stage. Experiments of 15N tracing at the reproductive stage showed, however, that the rate of N remobilization from the rosettes to the flowering organs and to the seeds was similar in early- and late-senescing lines. At the reproductive stage, N remobilization efficiency did not depend on senescence phenotypes but was related to the ratio between the biomasses of the sink and the source organs.

Original languageEnglish
Pages (from-to)1437-1449
Number of pages13
JournalPlant physiology
Volume147
Issue number3
DOIs
Publication statusPublished - Jul 1 2008

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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    Diaz, C., Lemaître, T., Christ, A., Azzopardi, M., Kato, Y., Sato, F., Morot-Gaudry, J. F., Le Dily, F., & Masclaux-Daubresse, C. (2008). Nitrogen recycling and remobilization are differentially controlled by leaf senescence and development stage in Arabidopsis under low nitrogen nutrition. Plant physiology, 147(3), 1437-1449. https://doi.org/10.1104/pp.108.119040