Nitrogen as a key regulator of flowering in Fagus crenata: Understanding the physiological mechanism of masting by gene expression analysis

Yuko Miyazaki, Yosuke Maruyama, Yukako Chiba, Masaki J. Kobayashi, Benesh Joseph, Kentaro K. Shimizu, Keiichi Mochida, Tsutom Hiura, Hirokazu Kon, Akiko Satake

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The role of resource availability in determining the incidence of masting has been widely studied, but how floral transition and initiation are regulated by the resource level is unclear. We tested the hypothesis that floral transition is stimulated by high resource availabiltiy in Fagus crenata based on a new technique, the expression analyses of flowering genes. We isolated F. crenata orthologues of FLOWERING LOCUS T, LEAFY and APETALA1, and confirmed their functions using transgenic Arabidopsis thaliana. We monitored the gene expression levels for 5 years and detected a cycle of on and offyears, which was correlated with fluctuations of the shoot-nitrogen concentration. Nitrogen fertilisation resulted in the significantly higher expression of flowering genes than the control, where all of the fertilised trees flowered, whereas the control did not. Our findings identified nitrogen as a key regulator of mast flowering, thereby providing new empirical evidence to support the resource budget model.

Original languageEnglish
Pages (from-to)1299-1309
Number of pages11
JournalEcology Letters
Volume17
Issue number10
DOIs
Publication statusPublished - 2014

Fingerprint

masting
Fagus
Fagus crenata
gene expression
flowering
Nitrogen
Gene Expression
nitrogen
resource
gene
Budgets
resource availability
Arabidopsis
Fertilization
genes
Arabidopsis thaliana
shoot
genetically modified organisms
incidence
shoots

Keywords

  • AP1
  • Floral initiation
  • Floral transition
  • Flowering
  • FT
  • Gene expression
  • Gene network
  • Intermittent reproduction
  • LFY
  • Life cycle
  • Resource budget model

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Medicine(all)

Cite this

Nitrogen as a key regulator of flowering in Fagus crenata : Understanding the physiological mechanism of masting by gene expression analysis. / Miyazaki, Yuko; Maruyama, Yosuke; Chiba, Yukako; Kobayashi, Masaki J.; Joseph, Benesh; Shimizu, Kentaro K.; Mochida, Keiichi; Hiura, Tsutom; Kon, Hirokazu; Satake, Akiko.

In: Ecology Letters, Vol. 17, No. 10, 2014, p. 1299-1309.

Research output: Contribution to journalArticle

Miyazaki, Yuko ; Maruyama, Yosuke ; Chiba, Yukako ; Kobayashi, Masaki J. ; Joseph, Benesh ; Shimizu, Kentaro K. ; Mochida, Keiichi ; Hiura, Tsutom ; Kon, Hirokazu ; Satake, Akiko. / Nitrogen as a key regulator of flowering in Fagus crenata : Understanding the physiological mechanism of masting by gene expression analysis. In: Ecology Letters. 2014 ; Vol. 17, No. 10. pp. 1299-1309.
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