Nitric oxide production by bronchoalveolar cells during allograft rejection in the rat

Tomoki Utsumi, Takatoshi Mizuta, Yoshitaka Fujii, Hiroyuki Shiono, Meinoshin Okumura, Masato Minami, Shin Ichi Takeda, Shinichiro Miyoshi, Hikaru Matsuda

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Backgrounds. We reported the increased nitric oxide (NO) level in exhaled air of rat lung transplant recipients during acute rejection (AR). The aim of this study was to determine the site and level of NO production in the rejected graft. Methods. Rat lung transplantation was performed in isografts and allografts. Results. In isografts, no AR and no significant increase in NO production was identified. In allografts, grades I-II of AR was seen on postoperative day (POD) 3 and grade III on POD 5. NO produced by BAL cells increased on both POD 3 (11.8±2.0 parts per billion [ppb]) and POD 5 (115.3±66.9 ppb). There was a highly significant correlation between the level of NO and the severity of AR (p=0.862, P <0.005). BAL cells from allografts expressed iNOS mRNA. Among them, macrophages, lymphocytes and neutrophils were immunostained for iNOS. Conclusion. NO produced by BAL cells was detected in the early stages of rejection. Therefore, it may serve as a sensitive indicator of AR in lung transplantation.

Original languageEnglish
Pages (from-to)1622-1626
Number of pages5
JournalTransplantation
Volume67
Issue number12
DOIs
Publication statusPublished - Jun 27 1999
Externally publishedYes

Fingerprint

Allografts
Nitric Oxide
Dimercaprol
Isografts
Lung Transplantation
Neutrophils
Macrophages
Air
Lymphocytes
Transplants
Lung
Messenger RNA

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Utsumi, T., Mizuta, T., Fujii, Y., Shiono, H., Okumura, M., Minami, M., ... Matsuda, H. (1999). Nitric oxide production by bronchoalveolar cells during allograft rejection in the rat. Transplantation, 67(12), 1622-1626. https://doi.org/10.1097/00007890-199906270-00019

Nitric oxide production by bronchoalveolar cells during allograft rejection in the rat. / Utsumi, Tomoki; Mizuta, Takatoshi; Fujii, Yoshitaka; Shiono, Hiroyuki; Okumura, Meinoshin; Minami, Masato; Takeda, Shin Ichi; Miyoshi, Shinichiro; Matsuda, Hikaru.

In: Transplantation, Vol. 67, No. 12, 27.06.1999, p. 1622-1626.

Research output: Contribution to journalArticle

Utsumi, T, Mizuta, T, Fujii, Y, Shiono, H, Okumura, M, Minami, M, Takeda, SI, Miyoshi, S & Matsuda, H 1999, 'Nitric oxide production by bronchoalveolar cells during allograft rejection in the rat', Transplantation, vol. 67, no. 12, pp. 1622-1626. https://doi.org/10.1097/00007890-199906270-00019
Utsumi T, Mizuta T, Fujii Y, Shiono H, Okumura M, Minami M et al. Nitric oxide production by bronchoalveolar cells during allograft rejection in the rat. Transplantation. 1999 Jun 27;67(12):1622-1626. https://doi.org/10.1097/00007890-199906270-00019
Utsumi, Tomoki ; Mizuta, Takatoshi ; Fujii, Yoshitaka ; Shiono, Hiroyuki ; Okumura, Meinoshin ; Minami, Masato ; Takeda, Shin Ichi ; Miyoshi, Shinichiro ; Matsuda, Hikaru. / Nitric oxide production by bronchoalveolar cells during allograft rejection in the rat. In: Transplantation. 1999 ; Vol. 67, No. 12. pp. 1622-1626.
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