Nitric oxide induces vascular endothelial growth factor expression in the rat placenta in vivo and in vitro

Hideaki Abe, Wataru Ishikawa, Takahiro Kushima, Tomoka Nishimura, Chiemi Mori, Atsushi Onuki, Takehito Suzuki, Yasuo Ishii, Norio Kansaku, Yoko Miyazaki, Kazuaki Tanaka, Hidetoshi Morita, Tatsuya Takizawa

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We investigated the role of nitric oxide (NO) in vascular endothelial growth factor (VEGF) expression in the rat placenta. A nitric oxide synthase (NOS) inhibitor, NG-nitro-L-arginine-methyl ester (L-NAME), was constantly infused into pregnant rats 6-24 h before sacrifice on gestational day (GD) 15.5. NO production declined to about 15% of the control level as monitored by NO trapping and electron paramagnetic resonance spectroscopy. VEGF mRNA expression was temporally decreased by L-NAME, but recovered to normal levels after 24 h of treatment, whereas hypoxia inducible factor (HIF)-1α and induced NOS (iNOS) expression increased. VEGF expression decreased significantly in placental explants after 6 h of co-treatment with LNAME and lipopolysaccharide, an iNOS inducer. Our data indicate that NO induce VEGF expression in vivo and in vitro in the rat placenta, suggesting that peaked NO production was maintained by a reciprocal relationship between NO and VEGF via HIF-1α.

Original languageEnglish
Pages (from-to)971-976
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume77
Issue number5
DOIs
Publication statusPublished - Jun 12 2013
Externally publishedYes

Keywords

  • Angiogenesis
  • Electron paramagnetic resonance (EPR) spectroscopy
  • Fetal rat placenta
  • Nitric oxide (NO)

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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  • Cite this

    Abe, H., Ishikawa, W., Kushima, T., Nishimura, T., Mori, C., Onuki, A., Suzuki, T., Ishii, Y., Kansaku, N., Miyazaki, Y., Tanaka, K., Morita, H., & Takizawa, T. (2013). Nitric oxide induces vascular endothelial growth factor expression in the rat placenta in vivo and in vitro. Bioscience, Biotechnology and Biochemistry, 77(5), 971-976. https://doi.org/10.1271/bbb.120923