Nitric oxide induces apoptosis in bovine luteal cells

Anna J. Korzekwa, Kiyoshi Okuda, Izabela Woclawek-Potocka, Shuko Murakami, Dariusz J. Skarzynski

Research output: Contribution to journalArticle

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Abstract

We previously showed in in vivo and in vitro studies that nitric oxide (NO) is engaged in luteolysis in cattle. Nitric oxide produced locally in the bovine corpus luteum (CL) inhibits progesterone (P4) synthesis and is suggested to be a component of the luteolytic cascade induced by uterine prostaglandin (PG) F. In the present study, the molecular mechanisms of NO action during structural luteolysis were studied in cultured bovine luteal cells (Days 15-17 of the estrous cycle). The effects of the NO donor (NONOate; 10-4M) on DNA fragmentation, cell viability, P4 production and caspase-3 activity were compared with those of PGF (10 -6M). Moreover, mobilization of intracellular calcium [Ca 2+]i and gene expressions of Fas-L, Fas, bcl-2, bax, and caspase-3 in the cells were determined by semi-quantitative RT-PCR after NONOate treatment. Caspase-3 activity was examined calorimetrically. Contrary to PGF NONOate decreased cell viability. DNA fragmentation after NONOate treatment increased by more than with PGF2. NONOate increased mobilization of [Ca2+]i in the cells. Although the NO donor did not affect Fas-L and bcl-2 gene expression, it stimulated Fas and bax mRNA and caspase-3 expression. The ratio of bcl-2 to bax mRNA level decreased in the cells treated with NONOate. Moreover, NONOate stimulated caspase-3 activity more effectively than PGF. The overall results suggest that NO is a luteolytic factor that plays a crucial role in regulation of the estrous cycle in structural luteolysis by inducing apoptosis of luteal cells in cattle.

Original languageEnglish
Pages (from-to)353-361
Number of pages9
JournalJournal of Reproduction and Development
Volume52
Issue number3
DOIs
Publication statusPublished - 2006

Fingerprint

Luteal Cells
luteal cells
Caspase 3
Dinoprost
nitric oxide
caspase-3
Nitric Oxide
Luteolysis
apoptosis
Apoptosis
luteolysis
cattle
Estrous Cycle
Nitric Oxide Donors
DNA Fragmentation
DNA fragmentation
Cell Survival
estrous cycle
cell viability
bcl-2 Genes

Keywords

  • Apoptosis
  • Cattle
  • Corpus luteum
  • Luteolysis
  • Nitric oxide

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine

Cite this

Korzekwa, A. J., Okuda, K., Woclawek-Potocka, I., Murakami, S., & Skarzynski, D. J. (2006). Nitric oxide induces apoptosis in bovine luteal cells. Journal of Reproduction and Development, 52(3), 353-361. https://doi.org/10.1262/jrd.17092

Nitric oxide induces apoptosis in bovine luteal cells. / Korzekwa, Anna J.; Okuda, Kiyoshi; Woclawek-Potocka, Izabela; Murakami, Shuko; Skarzynski, Dariusz J.

In: Journal of Reproduction and Development, Vol. 52, No. 3, 2006, p. 353-361.

Research output: Contribution to journalArticle

Korzekwa, AJ, Okuda, K, Woclawek-Potocka, I, Murakami, S & Skarzynski, DJ 2006, 'Nitric oxide induces apoptosis in bovine luteal cells', Journal of Reproduction and Development, vol. 52, no. 3, pp. 353-361. https://doi.org/10.1262/jrd.17092
Korzekwa AJ, Okuda K, Woclawek-Potocka I, Murakami S, Skarzynski DJ. Nitric oxide induces apoptosis in bovine luteal cells. Journal of Reproduction and Development. 2006;52(3):353-361. https://doi.org/10.1262/jrd.17092
Korzekwa, Anna J. ; Okuda, Kiyoshi ; Woclawek-Potocka, Izabela ; Murakami, Shuko ; Skarzynski, Dariusz J. / Nitric oxide induces apoptosis in bovine luteal cells. In: Journal of Reproduction and Development. 2006 ; Vol. 52, No. 3. pp. 353-361.
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