NIMA-related kinases 6, 4, and 5 interact with each other to regulate microtubule organization during epidermal cell expansion in Arabidopsis thaliana

Hiroyasu Motose, Takahiro Hamada, Kaori Yoshimoto, Takashi Murata, Mitsuyasu Hasebe, Yuichiro Watanabe, Takashi Hashimoto, Tatsuya Sakai, Taku Takahashi

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

NimA-related kinase 6 (NEK6) has been implicated in microtubule regulation to suppress the ectopic outgrowth of epidermal cells; however, its molecular functions remain to be elucidated. Here, we analyze the function of NEK6 and other members of the NEK family with regard to epidermal cell expansion and cortical microtubule organization. The functional NEK6-green fluorescent protein fusion localizes to cortical microtubules, predominantly in particles that exhibit dynamic movement along microtubules. The kinase-dead mutant of NEK6 (ibo1-1) exhibits a disturbance of the cortical microtubule array at the site of ectopic protrusions in epidermal cells. Pharmacological studies with microtubule inhibitors and quantitative analysis of microtubule dynamics indicate excessive stabilization of cortical microtubules in ibo1/nek6 mutants. In addition, NEK6 directly binds to microtubules in vitro and phosphorylates β-tubulin. NEK6 interacts and co-localizes with NEK4 and NEK5 in a transient expression assay. The ibo1-3 mutation markedly reduces the interaction between NEK6 and NEK4 and increases the interaction between NEK6 and NEK5. NEK4 and NEK5 are required for the ibo1/nek6 ectopic outgrowth phenotype in epidermal cells. These results demonstrate that NEK6 homodimerizes and forms heterodimers with NEK4 and NEK5 to regulate cortical microtubule organization possibly through the phosphorylation of β-tubulins.

Original languageEnglish
Pages (from-to)993-1005
Number of pages13
JournalPlant Journal
Volume67
Issue number6
DOIs
Publication statusPublished - Sep 2011

Fingerprint

Arabidopsis
Microtubules
microtubules
phosphotransferases (kinases)
Phosphotransferases
Arabidopsis thaliana
cells
Tubulin
tubulin
NIMA-related kinase 6
mutants
Green Fluorescent Proteins
green fluorescent protein
quantitative analysis
phosphorylation
Phosphorylation
Pharmacology
Phenotype
mutation
phenotype

Keywords

  • Arabidopsis thaliana
  • cell expansion
  • epidermal cell
  • microtubule
  • NimA-related kinase

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

Cite this

NIMA-related kinases 6, 4, and 5 interact with each other to regulate microtubule organization during epidermal cell expansion in Arabidopsis thaliana. / Motose, Hiroyasu; Hamada, Takahiro; Yoshimoto, Kaori; Murata, Takashi; Hasebe, Mitsuyasu; Watanabe, Yuichiro; Hashimoto, Takashi; Sakai, Tatsuya; Takahashi, Taku.

In: Plant Journal, Vol. 67, No. 6, 09.2011, p. 993-1005.

Research output: Contribution to journalArticle

Motose, Hiroyasu ; Hamada, Takahiro ; Yoshimoto, Kaori ; Murata, Takashi ; Hasebe, Mitsuyasu ; Watanabe, Yuichiro ; Hashimoto, Takashi ; Sakai, Tatsuya ; Takahashi, Taku. / NIMA-related kinases 6, 4, and 5 interact with each other to regulate microtubule organization during epidermal cell expansion in Arabidopsis thaliana. In: Plant Journal. 2011 ; Vol. 67, No. 6. pp. 993-1005.
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