NFATx, a novel member of the nuclear factor of activated T cells family that is expressed predominantly in the thymus

Esteban S. Masuda, Yoshiyuki Naito, Hiroshi Tokumitsu, Dave Campbell, Fumiko Saito, Charles Hannum, Ken Ichi Arai, Naoko Arai

Research output: Contribution to journalArticle

194 Citations (Scopus)

Abstract

The nuclear factor of activated T cells (NFAT) regulates cytokine gene expression in T cells through cis-acting elements located in the promoters of cytokine genes. Here, we report the cDNA cloning, chromosomal localization, and initial characterization of a transcription factor related to NFATp and NFATc. The novel molecule, designated NFATx, exhibits in its middle a region very similar to the Rel homology domain in NFATc and NFATp. The amino- terminal region of NFATx also shows significant similarities to corresponding sequences in NFATc and NFATp and contains three copies of a conspicuous 17- residue motif of unknown function. We provide evidence showing that NFATx can reconstitute binding to the NFAT-binding site from the interleukin 2 promoter when combined with AP1 (c-Fos/c-Jun) polypeptides and that NFATx is capable of activating transcription of the interleukin 2 promoter in COS-7 cells when stimulated with phorbol ester and calcium ionophore. NFATx mRNA is preferentially and remarkably found in the thymus and at lower levels in peripheral blood leukocytes. The expression pattern of NFATx, together with its functional activity, strongly suggests that NFATx plays a role in the regulation of gene expression in T cells and immature thymocytes.

Original languageEnglish
Pages (from-to)2697-2706
Number of pages10
JournalMolecular and Cellular Biology
Volume15
Issue number5
DOIs
Publication statusPublished - May 1995
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'NFATx, a novel member of the nuclear factor of activated T cells family that is expressed predominantly in the thymus'. Together they form a unique fingerprint.

  • Cite this