Newly mutated putative-aminotransferase in nonpungent pepper (Capsicum annuum) results in biosynthesis of capsinoids, capsaicinoid analogues

Yoshiyuki Tanaka, Munetaka Hosokawa, Tetsuya Miwa, Tatsuo Watanabe, Susumu Yazawa

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Capsinoids make up a group of nonpungent capsaicinoid analogues produced in Capsicum fruits. They have bioactivities similar to those of capsaicinoids such as suppression of fat accumulation and antioxidant activity. Because of their low pungency, they are more palatable ingredients in dietary supplements than capsaicinoids. We recently reported that capsinoid biosynthesis is caused by nonsense mutation in a putative aminotransferase gene (p-AMT) in a nonpungent cultivar CH-19 Sweet. Here we report on the screening of nonpungent germplasm that revealed a nonpungent cultivar Himo, which contains high levels of capsinoids. We have shown that Himo has a recessive allele of p-amt, which contains a mutation different from that of CH-19 Sweet. Sequence analysis of p-amt in Himo revealed that a single-nucleotide substitution results in one amino acid substitution from cysteine to arginine in the pyridoxal 5-phosphate binding domain. Genetic analysis using a cleaved amplified polymorphic sequence marker confirmed that the p-AMT genotype was precisely cosegregated with capsinoid biosynthesis and nonpungency. Himo will provide a new natural source of capsinoids.

Original languageEnglish
Pages (from-to)1761-1767
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number3
DOIs
Publication statusPublished - Feb 10 2010
Externally publishedYes

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Capsicum
Biosynthesis
transaminases
sweet peppers
Capsicum annuum
Transaminases
Substitution reactions
Dietary supplements
Genes
biosynthesis
germplasm screening
nonsense mutation
pyridoxal phosphate
Pyridoxal Phosphate
Nonsense Codon
cultivars
amino acid substitution
Amino Acid Substitution
Dietary Supplements
Fruits

Keywords

  • Capsicum
  • Capsinoid
  • Nonpungent capsaicinoid analogue
  • One-amino acid substitution
  • Putative aminotransferase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Newly mutated putative-aminotransferase in nonpungent pepper (Capsicum annuum) results in biosynthesis of capsinoids, capsaicinoid analogues. / Tanaka, Yoshiyuki; Hosokawa, Munetaka; Miwa, Tetsuya; Watanabe, Tatsuo; Yazawa, Susumu.

In: Journal of Agricultural and Food Chemistry, Vol. 58, No. 3, 10.02.2010, p. 1761-1767.

Research output: Contribution to journalArticle

Tanaka, Yoshiyuki ; Hosokawa, Munetaka ; Miwa, Tetsuya ; Watanabe, Tatsuo ; Yazawa, Susumu. / Newly mutated putative-aminotransferase in nonpungent pepper (Capsicum annuum) results in biosynthesis of capsinoids, capsaicinoid analogues. In: Journal of Agricultural and Food Chemistry. 2010 ; Vol. 58, No. 3. pp. 1761-1767.
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