New TNF-α releasing inhibitors, geraniin and corilagin, in leaves of Acer nikoense, Megusurino-ki

Sachiko Okabe, Masami Suganuma, Yoko Imayoshi, Shoko Taniguchi, Takashi Yoshida, Hirota Fujiki

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

The success of green tea as a cancer preventive is based on evidence that green tea contains tannins and antioxidants, does not show toxicity in humans and has long traditional use in Asia. In the light of this, herbal medicines are now also attracting attention as potential sources of cancer preventive agents. Using the inhibition of TNF-α release assay, we studied Acer nikoense (Megusurino-ki in Japanese), one of the herbal medicines. The inhibitory activity of TNF-α release was found in the leaf extract rather than the bark extract, and the main active constituents were identified as geraniin and corilagin, which are present in another Japanese traditional herb, Geranium thunbergii (Genno-shoko). The IC50 values of TNF-α release inhibition were 43 μM for geraniin and 76 μM for corilagin, whereas that for (-)-epigallocatechin gallate (EGCG) was 26 μM. Treatment with geraniin prior to application of okadaic acid, a tumor promoter on mouse skin initiated with 7,12-dimethylbenz(a)anthracene, reduced the percentage of tumor-bearing mice from 80.0 to 40.0% and the average numbers of tumor per mouse from 3.8 to 1.1 in week 20. Thus, geraniin has slightly weaker inhibitory activity than EGCG. Since geraniin and corilagin have been well investigated as representative tannins, we discuss here the new possibility of classical herbal medicine in the development of preventive agents for cancer and other life-style related diseases.

Original languageEnglish
Pages (from-to)1145-1148
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume24
Issue number10
DOIs
Publication statusPublished - 2001

Fingerprint

Acer
Herbal Medicine
Tannins
Tea
Neoplasms
Geranium
Okadaic Acid
Carcinogens
Inhibitory Concentration 50
Life Style
Antioxidants
corilagin
Geraniin
Skin

Keywords

  • Acer nikoense
  • Cancer chemoprevention
  • Genno-shoko
  • Green tea
  • Tannin
  • Tumor promotion

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

New TNF-α releasing inhibitors, geraniin and corilagin, in leaves of Acer nikoense, Megusurino-ki. / Okabe, Sachiko; Suganuma, Masami; Imayoshi, Yoko; Taniguchi, Shoko; Yoshida, Takashi; Fujiki, Hirota.

In: Biological and Pharmaceutical Bulletin, Vol. 24, No. 10, 2001, p. 1145-1148.

Research output: Contribution to journalArticle

Okabe, Sachiko ; Suganuma, Masami ; Imayoshi, Yoko ; Taniguchi, Shoko ; Yoshida, Takashi ; Fujiki, Hirota. / New TNF-α releasing inhibitors, geraniin and corilagin, in leaves of Acer nikoense, Megusurino-ki. In: Biological and Pharmaceutical Bulletin. 2001 ; Vol. 24, No. 10. pp. 1145-1148.
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