New role of glycosaminoglycans on the plasma membrane proposed by their interaction with phosphatidylcholine

Ayano Satoh, Toshihiko Toida, Keiichi Yoshida, Kyoko Kojima, Isamu Matsumoto

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Glycosaminoglycan side chains of membrane proteoglycans have been claimed to be located at the outermost layer of the glycocalyx surrounding the cell. In this study measurements by surface plasmon resonance and solid-phase assay have shown that both chondroitin sulfate and keratan sulfate but not heparin associate with phosphatidylcholine under physiological conditions. Spectrophotometric measurements also showed that chondroitin sulfate restricts the lateral diffusion of phosphatidylcholine in liposomes. These findings indicate that chondroitin sulfate and/or keratan sulfate chains of membrane proteoglycans crouch on the surface of the membrane while heparan sulfate chains stretch outward from the membrane surface as postulated traditionally. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)249-252
Number of pages4
JournalFEBS Letters
Volume477
Issue number3
DOIs
Publication statusPublished - Jul 21 2000
Externally publishedYes

Fingerprint

Cell membranes
Glycosaminoglycans
Phosphatidylcholines
Chondroitin Sulfates
Cell Membrane
Keratan Sulfate
Membranes
Proteoglycans
Glycocalyx
Heparitin Sulfate
Surface Plasmon Resonance
Surface plasmon resonance
Liposomes
Heparin
Assays

Keywords

  • Glycosaminoglycan
  • Membrane
  • Microdomain
  • Phospholipid

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

New role of glycosaminoglycans on the plasma membrane proposed by their interaction with phosphatidylcholine. / Satoh, Ayano; Toida, Toshihiko; Yoshida, Keiichi; Kojima, Kyoko; Matsumoto, Isamu.

In: FEBS Letters, Vol. 477, No. 3, 21.07.2000, p. 249-252.

Research output: Contribution to journalArticle

Satoh, Ayano ; Toida, Toshihiko ; Yoshida, Keiichi ; Kojima, Kyoko ; Matsumoto, Isamu. / New role of glycosaminoglycans on the plasma membrane proposed by their interaction with phosphatidylcholine. In: FEBS Letters. 2000 ; Vol. 477, No. 3. pp. 249-252.
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