New insights into the dimerization of small GTPase Rac/ROP guanine nucleotide exchange factors in rice

Akira Akamatsu, Kazumi Uno, Midori Kato, Hann Ling Wong, Ko Shimamoto, Yoji Kawano

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Molecular links between receptor-kinases and Rac/ROP family small GTPases mediated by activator guanine nucleotide exchange factors (GEFs) govern diverse biological processes. However, it is unclear how the Rac/ROP GTPases orchestrate such a wide variety of activities. Here, we show that rice OsRacGEF1 forms homodimers, and heterodimers with OsRacGEF2, at the plasma membrane (PM) and the endoplasmic reticulum (ER). OsRacGEF2 does not bind directly to the receptor-like kinase (RLK) OsCERK1, but forms a complex with OsCERK1 through OsRacGEF1 at the ER. This complex is transported from ER to the PM and there associates with OsRac1, resulting in the formation of a stable immune complex. Such RLK-GEF heterodimer complexes may explain the diversity of Rac/ROP family GTPase signalings.

Original languageEnglish
Article numbere1044702
JournalPlant Signaling and Behavior
Volume10
Issue number7
DOIs
Publication statusPublished - Jul 3 2015
Externally publishedYes

Keywords

  • PRONE GEF
  • Rac/ROP GTPase
  • dimerization
  • membrane trafficking

ASJC Scopus subject areas

  • Plant Science

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