New efficient replication system with hepatitis C virus genome derived from a patient with acute hepatitis C

Kyoko Mori, Ken ichi Abe, Hiromichi Dansako, Yasuo Ariumi, Masanori Ikeda, Nobuyuki Kato

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We report for the first time a new RNA replication system with a hepatitis C virus (HCV) strain (AH1) derived from a patient with acute hepatitis C. Using an HCV replicon RNA library constructed with the AH1 strain (genotype 1b), we first established a cloned cell line, sAH1, harboring the HCV replicon. Cured cells obtained with interferon treatment of sAH1 cells were used for transfection with genome-length HCV RNA possessing four mutations found in sAH1 replicon. Consequently, one cloned cell line, AH1, supporting efficient replication of genome-length HCV RNA was obtained. By the comparison of AH1 cells with the O cells supporting genome-length HCV RNA (HCV-O strain) replication, we found different anti-HCV profiles of interferon-γ and cyclosporine A between AH1 and O cells. Reporter assay analysis suggests that the diverse effects of interferon-γ are due to the difference in HCV strains, but not the cellular environment.

Original languageEnglish
Pages (from-to)104-109
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume371
Issue number1
DOIs
Publication statusPublished - Jun 20 2008

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Keywords

  • Acute hepatitis C
  • Anti-HCV reagents
  • Genome-length HCV RNA
  • HCV replication system
  • Hepatitis C virus
  • Interferon-γ

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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