New dot-blot method for evaluating the effect of inactivators on mite and Japanese cedar pollen allergens

Megumi Yoshida, Keijiro Mizukami, Keigo Kurata, Tadahiro Nasukawa, Jumpei Uchiyama, Masahiro Sakaguchi

Research output: Contribution to journalArticlepeer-review

Abstract

As a method of evaluating the effect of inactivators on allergens while suppressing the effect of inactivator on the assay, we developed new dot-blot method that combines immunostaining and protein detection methods. This method is useful for evaluating whether the inactivator can inactivate allergens rather than removing them from the assay.

Original languageEnglish
Pages (from-to)2089-2092
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume85
Issue number10
DOIs
Publication statusPublished - Sep 1 2021
Externally publishedYes

Keywords

  • Cry j 1
  • Der f 1
  • Dot-blot

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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