Neutrophil recruitment by intradermally injected neutrophil attractant/activation protein-1

Edward J. Leonard, Teizo Yoshimura, Shuji Tanaka, Mark Raffeld

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Neutrophil attractant/activation protein-1 (NAP-1) is a recently described cytokine that attracts neutrophils, but not monocytes or eosinophils. This leukocyte specificity is not absolute, in that NAP-1 attracts basophils and small numbers of lymphocytes. Our purpose was to determine in vivo effects of NAP-1, and to compare them to the reported action of the complement attractant, C5a. Intradermal injection into normal human subjects of 40 μl of NAP-1, over a concentration range of 4 × 10-8 M to 10-6 M, caused no symptoms or signs such as wheal-and-flare, itching, induration, or tenderness. However, biopsies of injection sites showed perivascular neutrophil infiltration as early as 30 min, which increased at 1 and 3 h. The mean number of neutrophils per mm2 of dermis for 15 biopsies taken 3 h after intradermal injection of 2 × 10-7 M or 10-6 M NAP-1 was 164±41; the response to saline or a NAP-1 inactive fragment was 5 or less. Intradermal NAP-1 did not cause basophil or lymphocyte infiltration. Consistent with the absence of a wheal-and-flare, acid toluidine blue-stained sections showed no evidence of mast cell degranulation, in contrast to previously reported results with C5a. Thus, the predominant response by human subjects to intradermal NAP-1 was neutrophil accumulation in proximity to dermal blood vessels.

Original languageEnglish
Pages (from-to)690-694
Number of pages5
JournalJournal of Investigative Dermatology
Volume96
Issue number5
Publication statusPublished - May 1991
Externally publishedYes

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Neutrophil Activation
Neutrophil Infiltration
Chemical activation
Proteins
Intradermal Injections
Neutrophils
Lymphocytes
Basophils
Biopsy
Infiltration
Complement C5a
Cell Degranulation
Tolonium Chloride
Blood vessels
Lymphocyte Count
Pruritus
Dermis
Eosinophils
Mast Cells
Signs and Symptoms

ASJC Scopus subject areas

  • Dermatology

Cite this

Neutrophil recruitment by intradermally injected neutrophil attractant/activation protein-1. / Leonard, Edward J.; Yoshimura, Teizo; Tanaka, Shuji; Raffeld, Mark.

In: Journal of Investigative Dermatology, Vol. 96, No. 5, 05.1991, p. 690-694.

Research output: Contribution to journalArticle

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