Neutrophil recruitment by intradermally injected neutrophil attractant/activation protein-1

Edward J. Leonard, Teizo Yoshimura, Shuji Tanaka, Mark Raffeld

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Abstract

Neutrophil attractant/activation protein-1 (NAP-1) is a recently described cytokine that attracts neutrophils, but not monocytes or eosinophils. This leukocyte specificity is not absolute, in that NAP-1 attracts basophils and small numbers of lymphocytes. Our purpose was to determine in vivo effects of NAP-1, and to compare them to the reported action of the complement attractant, C5a. Intradermal injection into normal human subjects of 40 μl of NAP-1, over a concentration range of 4 × 10-8 M to 10-6 M, caused no symptoms or signs such as wheal-and-flare, itching, induration, or tenderness. However, biopsies of injection sites showed perivascular neutrophil infiltration as early as 30 min, which increased at 1 and 3 h. The mean number of neutrophils per mm2 of dermis for 15 biopsies taken 3 h after intradermal injection of 2 × 10-7 M or 10-6 M NAP-1 was 164±41; the response to saline or a NAP-1 inactive fragment was 5 or less. Intradermal NAP-1 did not cause basophil or lymphocyte infiltration. Consistent with the absence of a wheal-and-flare, acid toluidine blue-stained sections showed no evidence of mast cell degranulation, in contrast to previously reported results with C5a. Thus, the predominant response by human subjects to intradermal NAP-1 was neutrophil accumulation in proximity to dermal blood vessels.

Original languageEnglish
Pages (from-to)690-694
Number of pages5
JournalJournal of Investigative Dermatology
Volume96
Issue number5
Publication statusPublished - May 1991
Externally publishedYes

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ASJC Scopus subject areas

  • Dermatology

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