Neutrophil infiltration as a crucial step for monocyte chemoattractant protein (MCP)-1 to attract monocytes in lipopolysaccharide-induced arthritis in rabbits

S. Miyazaki, A. Matsukawa, S. Ohkawara, K. Takagi, M. Yoshinaga

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objective and Design: To evaluate the mechanism whereby monocyte chemoattractant protein (MCP)-1 attracts monocytes in vivo. Subjects: New Zealand white rabbits (175 rabbits) were used. Treatment: LPS, MCP-1 or IL-8 was injected into knee joints. Antibodies against various cytokines or IL-1 receptor antagonist were injected to neutralize cytokine activities. Methods: The numbers of leukocyte populations, levels of cytokines in joints were estimated. Results: Partial inhibition of neutrophil influx with anti-IL-8 IgG (10 μg) suppressed LPS-induced macrophage influx by 43 ± 8.5% (p<0.05) without affecting the MCP-1 level. Intraarticular injection of MCP-1 (1-30 μg) induced macrophage influx. The event was accompanied by a small number of neutrophils in an early phase. Co-injection of IL-8 (1.0 μg) enhanced the MCP-1-induced macrophage infiltration (p<0.01). In neutrophil-depleted rabbits, LPS failed to induce macrophage influx even though the MCP-1 level was maintained, and macrophage influx following exogenously administered MCP-1 was also dramatically inhibited. Conclusions: Early events associated with neutrophil infiltration appear to be important for MCP-1 to induce a later macrophage influx in LPS-arthritis.

Original languageEnglish
Pages (from-to)673-678
Number of pages6
JournalInflammation Research
Volume49
Issue number12
DOIs
Publication statusPublished - Dec 1 2000
Externally publishedYes

Keywords

  • IL-8
  • LPS
  • MCP-1
  • Macrophage infiltration
  • Neutrophil infiltration

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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