Neutrophil attractant/activation protein-1 (NAP-1) causes human basophil histamine release

Martha V. White, Teizo Yoshimura, William Hook, Michael A. Kaliner, Edward J. Leonard

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Basophils from five of six human donors released histamine in response to neutrophil attractant/activation protein-1 (NAP-1). Histamine release by this protein was concentration-dependent over the range of 3×10-7 M to 4×10-6 M. At 4×10-6 M, the mean agonist-induced release was 16 ± 3% (SEM) of total basophil histamine. For the same basophil preparations, release by anti-IgE was 35 ± 6%. The chemotactic protein did not cause release of histamine from basophils at 0°C or in the presence of 10 mM EDTA. The time-course of histamine release was rapid; release was 43% of maximal after 30 s and maximal after 1 min of incubation. Thus, in addition to its previously characterized neutrophil chemotactic and activating properties, this protein activates human basophils.

Original languageEnglish
Pages (from-to)151-154
Number of pages4
JournalImmunology Letters
Volume22
Issue number2
DOIs
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

Neutrophil Activation
Basophils
Histamine Release
Proteins
Histamine
Edetic Acid
Neutrophils

Keywords

  • Basophils
  • Chemotaxis
  • Histamine release
  • Monocyte
  • Neutrophil
  • Neutrophil attractant/activation protein-1

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Neutrophil attractant/activation protein-1 (NAP-1) causes human basophil histamine release. / White, Martha V.; Yoshimura, Teizo; Hook, William; Kaliner, Michael A.; Leonard, Edward J.

In: Immunology Letters, Vol. 22, No. 2, 1989, p. 151-154.

Research output: Contribution to journalArticle

White, Martha V. ; Yoshimura, Teizo ; Hook, William ; Kaliner, Michael A. ; Leonard, Edward J. / Neutrophil attractant/activation protein-1 (NAP-1) causes human basophil histamine release. In: Immunology Letters. 1989 ; Vol. 22, No. 2. pp. 151-154.
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