Abstract
We recently found that normal human sera contain IgC antibodies against two chemoattractants, neutrophil attractant protein-1 (NAP-1/IL-8) and monocyte chemoattractant protein-1 (MCP-1), as well as immune complexes of these proteins. Intravenously administered LPS was reported to cause a sharp rise in serum NAP-1 concentration. Our study was designed to determine if LPS also caused an increase in MCP-1 and to measure associated changes in concentrations of antibody and immune complex. LPS caused a rise to a peak within 2 to 3 h in serum concentrations of free NAP-1 and MCP-1, followed by an almost equally rapid fall toward base-line levels by about 5 h postinjection. MCP-1 concentration in sera from the 11 subjects rose to a peak of 330 ± 52 pM. The peak value for NAP-1 was 80 ± 11 pM. In 10 of the 11 subjects, free IgG autoantibody to MCP-1 decreased from a mean pre-LPS value of 1820 ± 660 pM to a mean low of 53% of the respective initial values. Corresponding data for IgG anti-NAP-1 were a pre-LPS concentration of 216 ± 7 pM, which decreased to a mean low of 44% of the respective initial values. The finding in some subjects of a rapid rise in free antibody after the nadir suggests the possibility of acute regulation of autoantibody secretion rates. Although the results suggested that LPS-induced chemoattractant combined with free antibody, serum concentrations of MCP-1 -IgG or NAP-1 -IgG did not increase, which points to an as yet unknown mechanism for trapping and elimination of the immune complexes.
| Original language | English |
|---|---|
| Pages (from-to) | 3292-3298 |
| Number of pages | 7 |
| Journal | Journal of Immunology |
| Volume | 151 |
| Issue number | 6 |
| Publication status | Published - 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Neutrophil attractant protein-1 and monocyte chemoattractant protein-1 in human serum : Effects of intravenous lipopolysaccharide on free attractants, specific IgG autoantibodies and immune complexes. / Sylvester, Ilona; Suffredini, Anthony F.; Boujoukos, Arthur J.; Daniel Martich, G.; Danner, Robert L.; Yoshimura, Teizo; Leonard, Edward J.
In: Journal of Immunology, Vol. 151, No. 6, 1993, p. 3292-3298.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neutrophil attractant protein-1 and monocyte chemoattractant protein-1 in human serum
T2 - Effects of intravenous lipopolysaccharide on free attractants, specific IgG autoantibodies and immune complexes
AU - Sylvester, Ilona
AU - Suffredini, Anthony F.
AU - Boujoukos, Arthur J.
AU - Daniel Martich, G.
AU - Danner, Robert L.
AU - Yoshimura, Teizo
AU - Leonard, Edward J.
PY - 1993
Y1 - 1993
N2 - We recently found that normal human sera contain IgC antibodies against two chemoattractants, neutrophil attractant protein-1 (NAP-1/IL-8) and monocyte chemoattractant protein-1 (MCP-1), as well as immune complexes of these proteins. Intravenously administered LPS was reported to cause a sharp rise in serum NAP-1 concentration. Our study was designed to determine if LPS also caused an increase in MCP-1 and to measure associated changes in concentrations of antibody and immune complex. LPS caused a rise to a peak within 2 to 3 h in serum concentrations of free NAP-1 and MCP-1, followed by an almost equally rapid fall toward base-line levels by about 5 h postinjection. MCP-1 concentration in sera from the 11 subjects rose to a peak of 330 ± 52 pM. The peak value for NAP-1 was 80 ± 11 pM. In 10 of the 11 subjects, free IgG autoantibody to MCP-1 decreased from a mean pre-LPS value of 1820 ± 660 pM to a mean low of 53% of the respective initial values. Corresponding data for IgG anti-NAP-1 were a pre-LPS concentration of 216 ± 7 pM, which decreased to a mean low of 44% of the respective initial values. The finding in some subjects of a rapid rise in free antibody after the nadir suggests the possibility of acute regulation of autoantibody secretion rates. Although the results suggested that LPS-induced chemoattractant combined with free antibody, serum concentrations of MCP-1 -IgG or NAP-1 -IgG did not increase, which points to an as yet unknown mechanism for trapping and elimination of the immune complexes.
AB - We recently found that normal human sera contain IgC antibodies against two chemoattractants, neutrophil attractant protein-1 (NAP-1/IL-8) and monocyte chemoattractant protein-1 (MCP-1), as well as immune complexes of these proteins. Intravenously administered LPS was reported to cause a sharp rise in serum NAP-1 concentration. Our study was designed to determine if LPS also caused an increase in MCP-1 and to measure associated changes in concentrations of antibody and immune complex. LPS caused a rise to a peak within 2 to 3 h in serum concentrations of free NAP-1 and MCP-1, followed by an almost equally rapid fall toward base-line levels by about 5 h postinjection. MCP-1 concentration in sera from the 11 subjects rose to a peak of 330 ± 52 pM. The peak value for NAP-1 was 80 ± 11 pM. In 10 of the 11 subjects, free IgG autoantibody to MCP-1 decreased from a mean pre-LPS value of 1820 ± 660 pM to a mean low of 53% of the respective initial values. Corresponding data for IgG anti-NAP-1 were a pre-LPS concentration of 216 ± 7 pM, which decreased to a mean low of 44% of the respective initial values. The finding in some subjects of a rapid rise in free antibody after the nadir suggests the possibility of acute regulation of autoantibody secretion rates. Although the results suggested that LPS-induced chemoattractant combined with free antibody, serum concentrations of MCP-1 -IgG or NAP-1 -IgG did not increase, which points to an as yet unknown mechanism for trapping and elimination of the immune complexes.
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UR - http://www.scopus.com/inward/citedby.url?scp=0027317927&partnerID=8YFLogxK
M3 - Article
C2 - 8376779
AN - SCOPUS:0027317927
VL - 151
SP - 3292
EP - 3298
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 6
ER -