Neutrophil attractant protein-1 and monocyte chemoattractant protein-1 in human serum: Effects of intravenous lipopolysaccharide on free attractants, specific IgG autoantibodies and immune complexes

Ilona Sylvester, Anthony F. Suffredini, Arthur J. Boujoukos, G. Daniel Martich, Robert L. Danner, Teizo Yoshimura, Edward J. Leonard

Research output: Contribution to journalArticle

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Abstract

We recently found that normal human sera contain IgC antibodies against two chemoattractants, neutrophil attractant protein-1 (NAP-1/IL-8) and monocyte chemoattractant protein-1 (MCP-1), as well as immune complexes of these proteins. Intravenously administered LPS was reported to cause a sharp rise in serum NAP-1 concentration. Our study was designed to determine if LPS also caused an increase in MCP-1 and to measure associated changes in concentrations of antibody and immune complex. LPS caused a rise to a peak within 2 to 3 h in serum concentrations of free NAP-1 and MCP-1, followed by an almost equally rapid fall toward base-line levels by about 5 h postinjection. MCP-1 concentration in sera from the 11 subjects rose to a peak of 330 ± 52 pM. The peak value for NAP-1 was 80 ± 11 pM. In 10 of the 11 subjects, free IgG autoantibody to MCP-1 decreased from a mean pre-LPS value of 1820 ± 660 pM to a mean low of 53% of the respective initial values. Corresponding data for IgG anti-NAP-1 were a pre-LPS concentration of 216 ± 7 pM, which decreased to a mean low of 44% of the respective initial values. The finding in some subjects of a rapid rise in free antibody after the nadir suggests the possibility of acute regulation of autoantibody secretion rates. Although the results suggested that LPS-induced chemoattractant combined with free antibody, serum concentrations of MCP-1 -IgG or NAP-1 -IgG did not increase, which points to an as yet unknown mechanism for trapping and elimination of the immune complexes.

Original languageEnglish
Pages (from-to)3292-3298
Number of pages7
JournalJournal of Immunology
Volume151
Issue number6
Publication statusPublished - 1993
Externally publishedYes

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Chemokine CCL2
Antigen-Antibody Complex
Autoantibodies
Lipopolysaccharides
Neutrophils
Immunoglobulin G
Serum
Proteins
Antibodies
Chemotactic Factors
Interleukin-8
1-nitro-2-acetylpyrrole
human CCL2 protein

ASJC Scopus subject areas

  • Immunology

Cite this

Neutrophil attractant protein-1 and monocyte chemoattractant protein-1 in human serum : Effects of intravenous lipopolysaccharide on free attractants, specific IgG autoantibodies and immune complexes. / Sylvester, Ilona; Suffredini, Anthony F.; Boujoukos, Arthur J.; Daniel Martich, G.; Danner, Robert L.; Yoshimura, Teizo; Leonard, Edward J.

In: Journal of Immunology, Vol. 151, No. 6, 1993, p. 3292-3298.

Research output: Contribution to journalArticle

Sylvester, Ilona ; Suffredini, Anthony F. ; Boujoukos, Arthur J. ; Daniel Martich, G. ; Danner, Robert L. ; Yoshimura, Teizo ; Leonard, Edward J. / Neutrophil attractant protein-1 and monocyte chemoattractant protein-1 in human serum : Effects of intravenous lipopolysaccharide on free attractants, specific IgG autoantibodies and immune complexes. In: Journal of Immunology. 1993 ; Vol. 151, No. 6. pp. 3292-3298.
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